Human CD8 Magnetic Beads Kit

Host / Isotype

Mouse / Mouse IgG2a

Reactivity

Human

Application

Cell Separation

Conjugate

Magnetic Beads

CloneNo.

UCHT4

Cat no : KMS003

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Validation Data Gallery

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  Following depletion of CD8+ cells, supernatant cell suspension was stained with PB450-CD3(clone: HIT3a) and CL647-CD8(clone: OKT8). CD45+ cells are gated in the analysis. Left panel: CD3+CD8+ cells before selection. Right panel: CD3+CD8+ cells after depletion. Human CD8 magnetic beads kit is validated using PBMC from three different donors.

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Product Information

CD8 is a cell surface glycoprotein found on most cytotoxic T lymphocytes and is used as a marker for these cells. It acts as a coreceptor for T cell receptor and functions to recognize MHC –I antigens. 10%-30% human lymphocytes are CD8 positive. Human CD8 Magnetic Beads Kit is used for isolation or depletion of human CD8 T lymphocytes from PBMC, whole blood, or other sample types.  Following incubation with biotinylated human CD8 antibody and streptavidin conjugated magnetic beads, the cell sample is placed on a magnet. CD8+ cells remain attached to magnetic beads after separation and can be used for downstream applications, such as cell expansion, but are not suitable for flow cytometry analysis. CD8- cells remain in the supernatant and can be used for further applications.

Components

KMS003-10:   · MS001-10: 100µL 10mg/mL streptavidin magnetic beads   · MS65204-10: 100 µL 0.1mg/mL Biotin-CD8 (clone: UCHT4) KMS003-100:   · MS001-100: 1mL 10mg/mL streptavidin magnetic beads   · MS65204-100: 1mL 0.1mg/mL Biotin-CD8 (clone: UCHT4)

Storage

2-8°C

Storage buffer

Streptavidin beads: PBS, pH7.4, 0.2% BSA and 0.05% Sodium Azide

Biotin Antibody: PBS, pH7.4, 0.2% BSA and 0.09% Sodium Azide

Reactivity

Human

Recommend usage

10 µL Biotin-CD8 antibody and 10 µL streptavidin beads for 1*10⁷ cells

Cited in Article as

KMS003, Human CD8 Magnetic Beads Kit, Proteintech, IL, USA