• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Human CD4 Memory T Cell Isolation Kit

Reactivity

Human

Application

Cell Separation

Conjugate

Magnetic Beads

Cat no : KMS305

Validation Data Gallery

  • Following cell separation, cell suspension was stained with PB450-CD3 (UCHT1), FITC-CD4 (OKT4), PE-CD45RO (UCHL1) and CL647-CD45RA (HI100) antibodies. All viable cells are gated in the analysis. Left upper panel: CD3+CD4+ cells before selection; right upper panel: CD45RO+ cells within CD4+ cells. Left lower panel: CD3+CD4+ cells after selection; right lower panel: CD45RO+ cells within CD4+ cells. Human CD4 memory T cell isolation kit is tested using PBMC from three donors.

    Following cell separation, cell suspension was stained with PB450-CD3 (UCHT1), FITC-CD4 (OKT4), PE-CD45RO (UCHL1) and CL647-CD45RA (HI100) antibodies. All viable cells are gated in the analysis. Left upper panel: CD3+CD4+ cells before selection; right upper panel: CD45RO+ cells within CD4+ cells. Left lower panel: CD3+CD4+ cells after selection; right lower panel: CD45RO+ cells within CD4+ cells. Human CD4 memory T cell isolation kit is tested using PBMC from three donors.

Product Information

Memory T cells are antigen-exposed T lymphocytes which would mediate fast and robust response upon repeat encounter with antigen.  Human CD4 Memory T Cell Isolation kit is designed to isolate untouched CD4+ memory T lymphocytes which contains a biotin labeled antibody cocktail and streptavidin conjugated magnetic beads. Through incubation of samples with biotin antibody cocktail followed by incubation of streptavidin beads, the magnetically labeled non CD4+ memory cells will be retained in the tube by magnetic separator. The untouched CD4+ T memory cells are collected in supernatant. Any downstream application can be performed on CD4+ memory cells after separation, such as Flow Cytometry and in vitro culture.

Components

KMS305-10:  

   ·MS305A-10: 300 µL Human CD4 Isolation Biotin Antibody Cocktail (CD8, CD19, CD14, CD56, CD45RA, CD20, CD11b, CD16, CD36, CD235a, CD123, TCR γδ)

  ·MS305B-10: 300µL streptavidin magnetic beads  

KMS305-100:  

  ·MS305A-100: 3mL Human CD4 Isolation Biotin Antibody Cocktail (CD8, CD19, CD14, CD56, CD45RA, CD20, 11b, CD16, CD36, CD235a, CD123, TCR γδ)

  ·MS305B-100: 3mL streptavidin magnetic beads  


Storage

2-8°C

Storage buffer

PBS, pH7.4, 0.2% BSA and 0.05% Sodium Azide  

Reactivity

Human

Beads diameter

2.7 µm

Recommend usage

30µL biotin labeled antibody cocktail and 30µL streptavidin beads for 1x107 cells


Cited in Article as

KMS305, Human CD4 Memory T Cell Isolation Kit, Proteintech, IL, USA

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Matthias (Verified Customer) (07-04-2024)

The Kit worked well with primary leukocyte. Cell yield was as expected with a purity of 80-85% of the desired cell population

  • Applications: Flow Cytometry,Cell culture
  • Cell Tissue Type: primary Leukocytes