Human CD4 Magnetic Beads Kit

Host / Isotype

Mouse / IgG1

Reactivity

Human

Application

Cell Separation

Conjugate

Magnetic Beads

CloneNo.

RPA-T4

Cat no : KMS002

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Validation Data Gallery

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  Following depletion of CD4+ cells, supernatant cell suspension was stained with PB450-CD3(clone: HIT3a) and CL647-CD4(clone: OKT4). CD45+ cells are gated in the analysis. Left panel: CD3+CD4+ cells before selection. Right panel: CD3+CD4+ cells after depletion. Human CD4 magnetic beads kit is tested using PBMC from three donors.

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Product Information

CD4 is a coreceptor of T cell receptor in T lymphocytes to recognize antigens displayed by an antigen presenting cell in the context of class II MHC molecules. It is observed that 25%-65% human lymphocytes are CD4 positive. Human CD4 Magnetic Beads Kit is used for isolation or depletion of human CD4 T lymphocytes from PBMC, whole blood, or other sample types. Following incubation with biotinylated human CD4 antibody and Streptavidin magnetic beads, the cell sample is placed on a magnet.  CD4+ cells remain attached to magnetic beads after separation and can be used for downstream applications, such as cell expansion, but not suitable for flow cytometry. CD4- cells remain in supernatant and can also be used for further application.

Components

KMS002-10:

  · MS001-10: 100µL 10mg/mL streptavidin magnetic beads

  · MS65143-10: 100µL 0.1mg/mL Biotin-CD4 (clone: RPA-T4)

KMS002-100:

  · MS001-100: 1mL 10mg/mL streptavidin magnetic beads

  · MS65143-100: 1mL 0.1mg/mL Biotin-CD4 (clone: RPA-T4)

Storage

2-8°C

Storage buffer

Streptavidin beads: PBS, pH7.4, 0.2% BSA and 0.05% Sodium Azide

Biotin Antibody: PBS, pH7.4, 0.2% BSA and 0.09% Sodium Azide

Reactivity

Human

Recommend usage

10mL Biotin-CD4 antibody and 10mL streptavidin beads for 1*10⁷ cells

Cited in Article as

KMS002, Human CD4 Magnetic Beads Kit, Proteintech, IL, USA