Human CD4 Isolation Kit

Reactivity

Human

Application

Cell Separation

Conjugate

Magnetic Beads

Cat no : KMS302

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Validation Data Gallery

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  Following cell separation (enrichment), cell suspension was stained with FITC-CD45(F10-89-4), PE-CD3(UCHT1) and APC-CD4(OKT4) antibodies. All CD45+ cells are gated in the analysis. Left panel: CD3+CD4+ cells before selection. Right panel: CD3+CD4+ cells after selection. Human CD4 Isolation Kit is tested using PBMC from three donors.

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Product Information

CD4 is a 55 kDa glycoprotein that is a co-receptor for T cell receptor in T lymphocytes.  CD4+ cells are essential for achieving effective immune response by associating with class II MHC molecules.  25%-65% human lymphocytes are CD4+. Human CD4 Isolation Kit is designed to isolate untouched CD4+ T helper lymphocytes. It contains a biotin labeled antibody cocktail and streptavidin conjugated magnetic beads. Through incubation of samples with biotin labeled antibody cocktail followed by incubation of streptavidin beads, the magnetically labeled non CD4+ cells are retained in the tube by magnetic separator. The untouched CD4+ T helper cells are collected in supernatant. Any downstream application can be performed on CD4+ cells after separation, such as Flow Cytometry and in-vitro culture and expansion. 

Components

KMS302-10:  

  · MS302A-10: 300 µL Human CD4 Isolation Biotin Antibody Cocktail (CD8, CD19, CD14, CD56, CD20, CD11b, CD16, CD36, CD235a, CD123, TCR γδ)

  · MS302B-10: 300µL streptavidin magnetic beads  

KMS302-100:  

  · MS302A-100: 3mL Human CD4 Selection Biotin Antibody Cocktail (CD8, CD19, CD14, CD56, CD20, CD11b, CD16, CD36, CD235a, CD123, TCR γδ)

  · MS302B-100: 3mL streptavidin magnetic beads  

Storage

2-8°C

Storage buffer

PBS, pH7.4, 0.2% BSA and 0.05% Sodium Azide

Reactivity

Human 

Recommend usage

30µL biotin labeled antibody cocktail and 30µL streptavidin beads for 1*10⁷ cells.

Cited in Article as

KMS302, Human CD4 Isolation Kit, Proteintech, IL, USA