Human CD4 ELISA Kit
Cat no : KE00388
Synonyms
CD4,CD4 molecule,CD4mut,T-cell surface antigen T4/
Validation Data Gallery
Product Information
KE00388 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human CD4 ELISA kit is to be used to detect and quantify protein levels of endogenous human CD4. The assay recognizes human CD4. An antibody specific for human CD4 has been pre-coated onto the microwells. The human CD4 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human CD4 is added to detect the captured human CD4 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human CD4 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.3 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 920 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 75% | 71%-80% |
| Cell lysate | 103% | 84%-111% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 469.6 | 11.4 | 2.4 |
| 2 | 20 | 133.7 | 5.0 | 3.7 |
| 3 | 20 | 37.7 | 2.7 | 7.2 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 495.4 | 42.7 | 8.6 |
| 2 | 24 | 138.1 | 10.9 | 7.9 |
| 3 | 24 | 40.0 | 3.7 | 9.3 |
Background Information
CD4 is a transmembrane glycoproteinof the immunoglobulin super-family (IgSF), containing four extracellular Ig-like domains, ahydrophobic transmembrane region and a 40-residue cytoplasmictail. CD4 is expressed T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells.CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interactionand it is involved in T cell differentiation, development and activation by interacting with antigen-presenting cells (APCs). The cytoplasmic tail of CD4 associates with the Lck kinase, which in turn activates the signaling components of the T cell receptor (TCR)-CD3 complexes.CD4 also serves as a receptor for human immunodeficiency virus (HIV) by binding to the viral envelope glycoprotein gp120.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CD4,CD4 molecule,CD4mut,T-cell surface antigen T4/ |