Human CD19 Magnetic Beads Kit

Host / Isotype

Mouse / Mouse IgG1, kappa

Reactivity

Human

Application

Cell Separation

Conjugate

Magnetic Beads

CloneNo.

4G7

Cat no : KMS004

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Validation Data Gallery

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  Following depletion of CD19+cells, supernatant suspension was stained with PB450-CD3 (clone: HIT3a) and CL647-CD19 (cloneSJ25C). CD45+ cells are gated in the analysis. Left panel: CD3-CD19+ cells before selection. Right panel: CD3-CD19+ cells after depletion. Human CD19 magnetic bead kit is tested using PBMC from three different donors.

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Product Information

CD19 is a 95kDa, type I transmembrane glycoprotein member of the immunoglobin superfamily. It is expressed by B cells and follicular dendritic cells and regulates B-lineage commitment during hematopoietic stem cell differentiation. 6%-23% human PBMC are CD19 positive. Human CD19 Magnetic Beads Kit can be used for isolation or depletion of human CD19 B lymphocytes from PBMC, whole blood, or other sample types. Following incubation with biotinylated human CD19 antibody and Streptavidin magnetic beads, the cell sample is placed on a magnet. CD19+ cells remain attached to magnetic beads after separation and can be used for downstream applications, such as in expansion of cells, but are not suitable for flow cytometry analysis. CD19- cells remain in supernatant and can also be used for further applications.

Components

KMS004-10:   · MS001-10: 100µL 10mg/mL streptavidin magnetic beads   · MS65197-10: 100µL 0.1mg/mL Biotin-CD19 (clone: 4G7) KMS004-100:   · MS001-100: 1mL 10mg/mL streptavidin magnetic beads   · MS65197-100: 1mL 0.1mg/mL Biotin-CD19 (clone: 4G7)

Storage

2-8°C

Storage buffer

Streptavidin beads: PBS, pH7.4, 0.2% BSA and 0.05% Sodium Azide

Biotin Antibody: PBS, pH7.4, 0.2% BSA and 0.09% Sodium Azide

Reactivity

Human

Recommend usage

10µL Biotin-CD19 antibody and 10µL streptavidin beads for 1*10⁷ cells.

Cited in Article as

KMS004, Human CD19 Magnetic Beads Kit, Proteintech, IL, USA