Human CCL17 ELISA Kit
Cat no : KE00324
Synonyms
A 152E5.3, ABCD 2, C C motif chemokine 17, CC chemokine TARC, CCL17, SCYA17, Small inducible cytokine A17, TARC
Validation Data Gallery
Product Information
KE00324 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The CCL17 ELISA kit is to be used to detect and quantify protein levels of endogenous CCL17. The assay recognizes human CCL17. An antibody specific for CCL17 has been pre-coated onto the microwells. The CCL17 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-CCL17 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human CCL17 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Human plasma，Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 2.8 pg/mL |
| Range | 7.8-500 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 6361 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 112% | 96%-121% |
| Cell culture supernatants | 107% | 97%-126% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 253.3 | 13.2 | 5.2 |
| 2 | 20 | 55.8 | 3.4 | 6.1 |
| 3 | 20 | 11.9 | 1.1 | 9.1 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 260.0 | 8.1 | 3.1 |
| 2 | 24 | 60.1 | 2.3 | 3.8 |
| 3 | 24 | 15.1 | 1.1 | 7.4 |
Background Information
CCL17, also named as TARC, is a member of the CC chemokine family, and is highly expressed by thymus and other cells, including keratinocytes, endothelial cells, dendritic cells, bronchial epithelial cells and fibroblasts. CCL17 plays important roles in T cell development in thymus as well as in trafficking and activation of mature T cells. CCL17 induced chemotaxis and Ca2+ influx in the cells stably expressing CCR4, thereby demonstrating the ligand-receptor relationship between CCL17 and CCR4.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | A 152E5.3, ABCD 2, C C motif chemokine 17, CC chemokine TARC, CCL17, SCYA17, Small inducible cytokine A17, TARC |