Human Bcl-xL ELISA Kit
Cat no : KE00371
Synonyms
Validation Data Gallery
Product Information
KE00371 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human Bcl-xL ELISA kit is to be used to detect and quantify protein levels of endogenous human Bcl-xL. The assay recognizes human Bcl-xL. An antibody specific for human Bcl-xL has been pre-coated onto the microwells. The human Bcl-xL protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human Bcl-xL is added to detect the captured human Bcl-xL protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human Bcl-xL ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate, Tissue lysate |
| Assay type | Sandwich |
| Sensitivity | 1.6 pg/mL |
| Range | 78.1-5000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 598 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 96% | 78%-111% |
| Tissue lysate | 79% | 75%-82% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 2,826.7 | 157.5 | 5.6 |
| 2 | 20 | 610.7 | 19.1 | 3.1 |
| 3 | 20 | 207.4 | 9.7 | 4.7 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 2,684.2 | 113.2 | 4.2 |
| 2 | 24 | 645.4 | 21.9 | 3.4 |
| 3 | 24 | 211.4 | 12.3 | 5.8 |
Background Information
BCL2L1 is a member of the BCL-2 protein family. BCL2L1 is expressed as three isoforms, Bcl-xL, Bcl-xS, and Bcl-xβ, and is located at the outer mitochondrial membrane. The Bcl-xL isoform is a 233 amino acid protein, acting as an apoptotic inhibitor. Bcl-xL can form heterodimers with BAX, BAK or BCL2, and Bcl-xL regulates apoptosis by heterodimerization-dependent and -independent mechanisms.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms |