Human Angiopoietin 1 ELISA Kit
Cat no : KE00212
Synonyms
AGP1, AGPT, ANG 1, ANG1, angiopoietin 1, Angiopoietin-1, ANGPT1, KIAA0003, PDGFAB
Validation Data Gallery
Product Information
KE00212 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Angiopoietin 1 ELISA kit is to be used to detect and quantify protein levels of endogenous Angiopoietin 1. The assay recognizes human Angiopoietin 1. An antibody specific for Angiopoietin 1 has been pre-coated onto the microwells. The Angiopoietin 1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human Angiopoietin 1 is added to detect the captured human Angiopoietin 1 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human Angiopoietin 1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Saliva |
| Assay type | Sandwich |
| Sensitivity | 17.3 pg/mL |
| Range | 62.5 - 4000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 284 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 98% | 80%-116% |
| Human saliva | 89% | 71%-109% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 2,021.5 | 94.5 | 4.7 |
| 2 | 20 | 557.8 | 13.4 | 2.4 |
| 3 | 20 | 155.8 | 6.6 | 4.2 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 1,845.2 | 59.9 | 3.2 |
| 2 | 24 | 544.9 | 20.9 | 3.8 |
| 3 | 24 | 161.1 | 5.9 | 3.6 |
Background Information
Angiopoietin 1 is a 70-kDa secreted glycoprotein generated from vascular mural cells, pericytes, and certain other cells. Angiopoietin 1 participates in vascular differentiation through angiogenesis, which is the process of the growth and remodelling of existing vessels. Angiopoietin 1 is also involved in the maintenance and turnover of blood vessels in mature animals. Angiopoietin1 binds to and activates the TEK/TIE2 receptor by inducing its dimerization and tyrosine phosphorylation, playing an important role in the regulation of angiogenesis. Overexpression of Angiopoietin1 has been proven to occur in malignant glioblastoma, neuroblastoma, non-small cell lung cancer and other tumors.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | AGP1, AGPT, ANG 1, ANG1, angiopoietin 1, Angiopoietin-1, ANGPT1, KIAA0003, PDGFAB |
Publications
| Species | Sample Type | Title |
|---|---|---|
Acta Biomater Targeted Long-term Noninvasive Treatment of Choroidal Neovascularization by Biodegradable Nanoparticles |