Human Adiponectin ELISA Kit
Cat no : KE00080
Synonyms
ACDC, ACRP30, adiponectin, ADIPOQ, ADIPQTL1, ADPN, APM 1, APM1, GBP28, Gelatin binding protein
Validation Data Gallery
Product Information
KE00080 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Adiponectin ELISA kit is to be used to detect and quantify protein levels of endogenous Adiponectin. The assay recognizes human total Adiponectin (low, middle, and high molecular weight). An antibody specific for Adiponectin has been pre-coated onto the microwells. The Adiponectin protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for Adiponectin is added to detect the captured Adiponectin protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human Adiponectin ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants, Cell lysates |
| Assay type | Sandwich |
| Sensitivity | 24.0 pg/mL |
| Range | 62.5-4000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 9370 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 95% | 69%-116% |
| Cell culture supernatants | 100% | 81%-115% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 829.0 | 76.7 | 9.3 |
| 2 | 20 | 1,708.0 | 164.5 | 9.6 |
| 3 | 20 | 2,354.9 | 158.3 | 6.7 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 681.7 | 58.0 | 8.5 |
| 2 | 24 | 1,421.9 | 138.0 | 9.7 |
| 3 | 24 | 2,467.6 | 156.2 | 6.3 |
Background Information
Adiponectin (AdipoQ), an adipocyte-derived hormone, is one of the most abundant adipokines in the blood circulation. Human adiponectin exists in three multimer forms, high-molecular-weight (HMW), middle-molecular-weight (MMW), and low-molecular-weight (LMW). Adiponectin modulates a number of metabolic processes, including improving insulin sensitivity and anti-inflammatory activity. The role of Adiponectin in reproduction is not yet fully understood, but the expression of Adiponectin in reproductive tissues has been observed in various animals and humans, including chicken testis, bovine ovary, and human placenta. Adiponectin exerts its effects by activating a range of different signaling molecules via binding to two transmembrane Adiponectin receptors, AdipoR1 and AdipoR2. AdipoR1 is expressed primarily in the skeletal muscle, whereas AdipoR2 is predominantly expressed in the liver. Adiponectin May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | ACDC, ACRP30, adiponectin, ADIPOQ, ADIPQTL1, ADPN, APM 1, APM1, GBP28, Gelatin binding protein |
Publications
| Species | Sample Type | Title |
|---|---|---|
Am J Transplant Enhanced autophagy alleviated corneal allograft rejection via inhibiting NLRP3 inflammasome activity. | ||
J Clin Med Associations between RNA-Binding Motif Protein 3, Fibroblast Growth Factor 21, and Clinical Outcome in Patients with Stroke. | ||
J Food Biochem Attenuation of lipid accumulation in Bel-7402 cells through ADPN/AMPKα signaling stimulated by Fructus rosae laxae extract. | ||
Gynecol Endocrinol Forkhead box C2 is associated with insulin resistance in gestational diabetes mellitus. | ||
Biomolecules Development of a Vascularized Human Skin Equivalent with Hypodermis for Photoaging Studies | ||
Cells The Influence of Adipocyte Secretome on Selected Metabolic Fingerprints of Breast Cancer Cell Lines Representing the Four Major Breast Cancer Subtypes |