Human ANGPTL4 ELISA Kit
Cat no : KE00186
Synonyms
angiopoietin like 4, Angiopoietin like protein 4, Angiopoietin related protein 4, ANGPTL4, ARP4, FIAF, HFARP, PGAR, UNQ171/PRO197
Validation Data Gallery
Product Information
KE00186 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The ANGPTL4 ELISA kit is to be used to detect and quantify protein levels of endogenous ANGPTL4. The assay recognizes human ANGPTL4. An antibody specific for ANGPTL4 has been pre-coated onto the microwells. The ANGPTL4 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for ANGPTL4 is added to detect the captured ANGPTL4 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm .
| Product name | Human ANGPTL4 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma |
| Assay type | Sandwich |
| Sensitivity | 0.03 ng/mL |
| Range | 0.313-20 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 51129 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 94% | 74%-116% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 13.7 | 0.4 | 3.2 |
| 2 | 20 | 3.4 | 0.1 | 4.2 |
| 3 | 20 | 1.4 | 0.1 | 9.4 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 14.4 | 0.4 | 3.0 |
| 2 | 24 | 3.6 | 0.2 | 5.6 |
| 3 | 24 | 1.5 | 0.2 | 10.7 |
Background Information
Angiopoietin-like protein 4 (ANGPTL4), a member of the angiopoietin-like protein subfamily, is a secretory protein that functions as an important modulator of glucose and lipid metabolism. ANGPTL4 inhibits lipoprotein lipase (LPL)-dependent lipolysis thereby limiting the uptake of free fatty acids. Overexpression of ANGPTL4 results in hypertriglyceridemia, while ANGPTL4 deficiency suppresses foam formation in macrophages and protects against atherosclerosis. ANGPTL4 may play a role in several cancers and it also has been shown to prevent the metastatic process by inhibiting vascular activity as well as tumor cell motility and invasiveness. Decreased expression of this protein has been associated with type 2 diabete. Serum ANGPTL4 level might be a potential biomarker in renal cell carcinoma.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | angiopoietin like 4, Angiopoietin like protein 4, Angiopoietin related protein 4, ANGPTL4, ARP4, FIAF, HFARP, PGAR, UNQ171/PRO197 |