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Histone H3 Recombinant antibody, PBS Only

Histone H3 Uni-rAbTM Recombinant Antibody for IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1L5

Cat no : 82832-6-PBS

Synonyms

HIST1H3A, Histone-H3, HistoneH3, Histone H 3, 1L5

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Histone H3 Recombinant antibody (82832-6-RR)

    IF Staining of HeLa using 82832-6-RR (same clone as 82832-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Histone H3 antibody (82832-6-RR, Clone: 1L5 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82832-6-PBS in a different storage buffer formulation.

Product Information

82832-6-PBS targets Histone H3 in IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide
Full Name histone cluster 1, H3a
GenBank Accession NumberBC066245
Gene Symbol HIST1H3A
Gene ID (NCBI) 8350
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Histones are small, highly basic proteins that consist of a globular domain with unstructured N- and C-terminal tails protruding from the main structure. Histone H3 is one of the five main histones that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. In addition to their role in DNA compartmentalization, histones also play crucial roles in various biologic processes, including gene expression and regulation, DNA repair, chromatin condensation, cell cycle progression, chromosome segregation, and apoptosis. The ability of histones to regulate chromatin dynamics primarily originates from various posttranslational modifications carried out by histone-modifying enzymes.