Haptoglobin Polyclonal antibody

Haptoglobin Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, pig, rabbit

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 30412-1-AP

Synonyms

HP, Zonulin, HPA1S, HP2 ALPHA 2, HAPT

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Validation Data Gallery

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  WB analysis using 30412-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30412-1-AP (Haptoglobin antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 30412-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30412-1-AP (Haptoglobin antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of human plasma using 30412-1-AP

  IP result of anti-Haptoglobin (IP:30412-1-AP, 4ug; Detection:30412-1-AP 1:3000) with human plasma lysate 1600 ug.

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Tested Applications

• Positive WB detected in: rabbit blood tissue, pig plasma, rabbit plasma
• Positive IP detected in: human plasma

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30412-1-AP targets Haptoglobin in WB, IP, ELISA applications and shows reactivity with human, rat, pig, rabbit samples.

• Tested Reactivity: human, rat, pig, rabbit
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: haptoglobin
• Observed Molecular Weight: 36-45 kDa
• GenBank Accession Number: BC121125
• Gene Symbol: HP
• Gene ID (NCBI): 3240
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Haptoglobin is also named as Zonulin and belongs to the peptidase S1 family. Haptoglobin is an alpha-2-glycoprotein synthesized mainly by hepatocytes consisting of both alpha and beta subunits linked by disulfide bonds forming dimers (PMID: 4018023). Haptoglobin is primarily produced in the liver, and when red cells are lysed in vivo, free hemoglobin binds to circulating haptoglobin; the hemoglobin-haptoglobin complex is then degraded by the reticuloendothelial system (PMID: 24809098). Because haptoglobin levels become depleted in the presence of large amounts of free hemoglobin, decreased haptoglobin is a marker of hemolysis. The human haptoglobin (HP) gene exists in two major allelic forms leading to three major genotypes: Hp1-1, Hp2-1, and Hp-2-2, that separate on alkaline starch gel electrophoresis due to different molecular weights (PMID: 17474882). Their properties vary in binding strength, anti-oxidative ability, and rate of clearance. Binding strength is greatest with Hp1-1, but the Hp-2 genotypes can bind a larger number of hemoglobin alpha-beta dimers. Complexes of Hp2-2 and hemoglobin have higher affinity for the CD163 receptor, the monocyte-macrophage receptor that facilitates its clearance. Neutrophils contained a large amount of highly glycosylated Hp (β-chain 45-65 kDa) synthesized in neutrophil precursors and stored in specific granules and a small amount of Hp (β-chain 39 kDa) endocytosed from plasma and stored in secretory vesicles (PMID: 16543473).