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  • KD/KO Validated

HUWE1 Polyclonal antibody

HUWE1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 19430-1-AP

Synonyms

ARF binding protein 1, ARF BP1, HECTH9, HSPC272, HUWE1, Ib772, KIAA0312, KIAA1578, Large structure of UREB1, LASU1, Mcl 1 ubiquitin ligase E3, MULE, URE B1, URE binding protein 1, UREB1



Tested Applications

Positive WB detected inDaudi cells, HEK-293 cells
Positive IHC detected inhuman lung cancer tissue, human lung tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

19430-1-AP targets HUWE1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HUWE1 fusion protein Ag13763
Full Name HECT, UBA and WWE domain containing 1
Calculated molecular weight 437 aa, 482 kDa
Observed molecular weight 482 kDa
GenBank accession numberBC002602
Gene symbol HUWE1
Gene ID (NCBI) 10075
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HUWE1 encodes a HECT domain ubiquitin ligase which is a large protein (500 kDa) has attracted considerable interest because several and quite disparate substrates have been assigned to this E3. It has a role in regulating Berg-mann glia differentiation and this ubiquitin ligase orchestrates the programming of the neural progenitors that give rise to neurons and glia in the cerebellum. HUWE1 is essential for proliferation of a subset of tumor cells, and negative regulator of TP53 during the colorectal carcinoma progression through the ubiquitination pathway mediated by the HECT domain (PMID:15567145). HUWE1 plays a critical role in lung cancer and increased HUWE1 expression is significantly associated with worse prognosis which suggest that HUWE1 might be a potential target for lung cancer therapy (PMID: 30026863).

Protocols

Product Specific Protocols
WB protocol for HUWE1 antibody 19430-1-APDownload protocol
IHC protocol for HUWE1 antibody 19430-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cell

WNK1 is an assembly factor for the human ER membrane protein complex.

Authors - Tino Pleiner
humanWB

Sci Adv

Genomic gain of RRS1 promotes hepatocellular carcinoma through reducing the RPL11-MDM2-p53 signaling.

Authors - Pengbo Cao
  • KD Validated
human,mouseWB

FASEB J

HUWE1 promotes EGFR ubiquitination and degradation to protect against renal tubulointerstitial fibrosis.

Authors - Qian Zhu
humanWB

J Cell Sci

Derlin-1 promotes ubiquitination and degradation of epithelial sodium channel (ENaC).

Authors - Hui You
  • KD Validated
humanWB,IP

J Cell Sci

The role of USP7 in the Shoc2-ERK1/2 signaling axis and Noonan-like syndrome with loose anagen hair.

Authors - Patricia Wilson
  • KD Validated
humanWB

Biochem Biophys Res Commun

NEDD4L-mediated LTF protein degradation limits ferroptosis.

Authors - Yuan Wang