Tested Applications
| Positive WB detected in | BxPC-3 cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:300-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 5 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
Product Information
11223-1-AP targets HUS1 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1744 Product name: Recombinant human HUS1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-280 aa of BC007013 Sequence: MKFRAKIVDGACLNHFTRISNMIAKLAKTCTLRISPDKLNFILCDKLANGGVSMWCELEQENFFNEFQMEGVSAENNEIYLELTSENLSRALKTAQNARALKIKLTNKHFPCLTVSVELLSMSSSSRIVTHDIPIKVIPRKLWKDLQEPVVPDPDVSIYLPVLKTMKSVVEKMKNISNHLVIEANLDGELNLKIETELVCVTTHFKDLGNPPLASESTHEDRNVEHMAEVHIDIRKLLQFLAGQQVNPTKALCNIVNNKMVHFDLLHEDVSLQYFIPALS Predict reactive species |
| Full Name | HUS1 checkpoint homolog (S. pombe) |
| Calculated Molecular Weight | 32 kDa |
| Observed Molecular Weight | 32 kDa |
| GenBank Accession Number | BC007013 |
| Gene Symbol | HUS1 |
| Gene ID (NCBI) | 3364 |
| RRID | AB_2248839 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O60921 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Hus1 belongs to the Rad family, form a heterotrimeric clamp with Rad1and Rad9 that acts as a putative sensor for DNA damage. This complex plays an apical role in the DNA damage response, loss of a functional complex affects many downstream checkpoint pathways [PMID: 11340080]. It is also a key complex involved in the activation of the ATR pathway. 9-1-1 forms a ring-shaped heterotrimer, resembling the homotrimeric sliding clamp, proliferating cell nuclear antigen (PCNA) [PMID: 19535328].
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for HUS1 antibody 11223-1-AP | Download protocol |
| WB protocol for HUS1 antibody 11223-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Proc Natl Acad Sci U S A Uncoupling DNA damage from chromatin damage to detoxify doxorubicin. | ||
Oncogene DNA damage-induced translocation of mitochondrial factor HIGD1A into the nucleus regulates homologous recombination and radio/chemo-sensitivity. | ||
J Mol Cell Biol SUMO-1 modification of FEN1 facilitates its interaction with Rad9-Rad1-Hus1 to counteract DNA replication stress. | ||
Mol Carcinog HUS1 checkpoint clamp component (HUS1) is a potential tumor suppressor in primary hepatocellular carcinoma.
| ||
Am J Physiol Cell Physiol Succinylation at a key residue of FEN1 is involved in the DNA damage response to maintain genome stability. | ||
Dis Markers Discovery and Validation of Novel Methylation Markers in Helicobacter pylori-Associated Gastric Cancer. |
