HUS1 Polyclonal antibody

• Featured Product
• KD/KO Validated

HUS1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 11223-1-AP

Synonyms

Checkpoint protein HUS1, hHUS1, HUS1

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Validation Data Gallery

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  WB analysis of BxPC-3 using 11223-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 11223-1-AP (HUS1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 11223-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11223-1-AP (HUS1 antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive WB detected in: BxPC-3 cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:300-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

11223-1-AP targets HUS1 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HUS1 fusion protein Ag1744
• Full Name: HUS1 checkpoint homolog (S. pombe)
• Calculated Molecular Weight: 32 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC007013
• Gene Symbol: HUS1
• Gene ID (NCBI): 3364
• RRID: AB_2248839
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Hus1 belongs to the Rad family, form a heterotrimeric clamp with Rad1and Rad9 that acts as a putative sensor for DNA damage. This complex plays an apical role in the DNA damage response, loss of a functional complex affects many downstream checkpoint pathways [PMID: 11340080]. It is also a key complex involved in the activation of the ATR pathway. 9-1-1 forms a ring-shaped heterotrimer, resembling the homotrimeric sliding clamp, proliferating cell nuclear antigen (PCNA) [PMID: 19535328].

Publications

Species	Application	Title
human	WB	Oncogene; DNA damage-induced translocation of mitochondrial factor HIGD1A into the nucleus regulates homologous recombination and radio/chemo-sensitivity.; Authors - Bin Chen
human	WB	J Mol Cell Biol; SUMO-1 modification of FEN1 facilitates its interaction with Rad9-Rad1-Hus1 to counteract DNA replication stress.; Authors - Xiaoli Xu
human	WB,IHC	Mol Carcinog; HUS1 checkpoint clamp component (HUS1) is a potential tumor suppressor in primary hepatocellular carcinoma.; Authors - Zi-Qi Zhou ; KD Validated
human	IF	Am J Physiol Cell Physiol; Succinylation at a key residue of FEN1 is involved in the DNA damage response to maintain genome stability.; Authors - Rongyi Shi
human	WB	Dis Markers; Discovery and Validation of Novel Methylation Markers in Helicobacter pylori-Associated Gastric Cancer.; Authors - Huan Wang
		bioRxiv; USP50 suppresses alternative RecQ helicase use and deleterious DNA2 activity during replication; Authors - Hannah L Mackay