CoraLite®594-conjugated HSPH1 Monoclonal antibody

HSPH1 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

3H5A5

Cat no : CL594-66723

Synonyms

Antigen NY CO 25, DKFZp686M05240, Heat shock 110 kDa protein, Heat shock protein 105 kDa, HSP105, HSP105A, HSP105B, HSP110, HSPH1, KIAA0201, NY CO 25

---

Validation Data Gallery

• 

  IF Staining of HeLa using CL594-66723

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite®594 HSPH1 antibody (CL594-66723, Clone: 3H5A5 ) at dilution of 1:200.

• 

  IF Staining of HeLa using CL594-66723

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CL594-66723 (HSPH1 antibody) at dilution of 1:100.

---

Tested Applications

• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66723 targets HSPH1 in IF/ICC applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: HSPH1 fusion protein Ag27521
• Full Name: heat shock 105kDa/110kDa protein 1
• Calculated Molecular Weight: 858 aa, 97 kDa
• Observed Molecular Weight: 110 kDa
• GenBank Accession Number: BC037553
• Gene Symbol: HSPH1
• Gene ID (NCBI): 10808
• RRID: AB_2883618
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

HSP105, also known as HSP110 or HSPH1, belongs to the heat shock protein (HSP) family. Human HSP105 is a high-molecular-weight chaperone protein expressed at constitutively low levels as a cytoplasmic α-isoform and as an inducible nuclear β-isoform on exposure to various forms of stress. HSP105 is constitutively overexpressed in several solid tumors, including melanoma, breast, thyroid, and gastroenteric cancers, and exerts antiapoptotic functions. Recently HSP105 has been identified as a novel candidate biomarker of lymphoma aggressiveness. Western blot analysis using this antibody detected the phosphorylated protein around 110 kDa in various lysates.