HSPH1 Polyclonal antibody

HSPH1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 13383-1-AP

Synonyms

KIAA0201, HSP110, HSP105, Heat shock protein family H member 1, Antigen NY-CO-25

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Validation Data Gallery

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  WB analysis of MCF-7 using 13383-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 13383-1-AP (HSPH1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 13383-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13383-1-AP (HSPH1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 13383-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13383-1-AP (HSPH1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 13383-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13383-1-AP (HSPH1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 13383-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 13383-1-AP (HSPH1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 13383-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13383-1-AP (HSPH1 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 13383-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 13383-1-AP (HSPH1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 13383-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 13383-1-AP (HSPH1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver cancer using 13383-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13383-1-AP (HSPH1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 13383-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13383-1-AP (HSPH1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon cancer using 13383-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13383-1-AP (HSPH1 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human colon cancer using 13383-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13383-1-AP (HSPH1 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human liver cancer using 13383-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13383-1-AP (HSPH1 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver cancer using 13383-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13383-1-AP (HSPH1 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human pancreas cancer using 13383-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 13383-1-AP (HSPH1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas cancer using 13383-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 13383-1-AP (HSPH1 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: MCF7 cells, HeLa cells, human brain tissue, Jurkat cells, K-562 cells
• Positive IHC detected in: human colon cancer tissue, human testis tissue, human liver cancer tissue, human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:200-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IF: See 4 publications below

Product Information

13383-1-AP targets HSPH1 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HSPH1 fusion protein Ag4224
• Full Name: heat shock 105kDa/110kDa protein 1
• Calculated Molecular Weight: 858 aa, 97 kDa
• Observed Molecular Weight: 110 kDa
• GenBank Accession Number: BC037553
• Gene Symbol: HSPH1
• Gene ID (NCBI): 10808
• RRID: AB_2233157
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HSP105, also known as HSP110 or HSPH1, belongs to the heat shock protein (HSP) family. Human HSP105 is a high-molecular-weight chaperone protein expressed at constitutively low levels as a cytoplasmic α-isoform and as an inducible nuclear β-isoform on exposure to various forms of stress. HSP105 is constitutively overexpressed in several solid tumors, including melanoma, breast, thyroid, and gastroenteric cancers, and exerts antiapoptotic functions. Recently HSP105 has been identified as a novel candidate biomarker of lymphoma aggressiveness. This antibody recognizes both HSP105α and HSP105β isoforms. Western blot analysis using this antibody detected a major band around 100-110 kDa in Jurkat cells.

Publications

Species	Application	Title
human	IF	Nat Cell Biol; Heat-shock chaperone HSPB1 regulates cytoplasmic TDP-43 phase separation and liquid-to-gel transition; Authors - Shan Lu
mouse	WB	Mol Ther Nucleic Acids; Age-associated changes in microglia and astrocytes ameliorate blood-brain barrier dysfunction; Authors - Jie Pan
mouse	WB	Mol Reprod Dev; Identification of Hsf1 as a novel androgen receptor-regulated gene in mouse sertoli cells.; Authors - Lihua Yang
human	IF	bioRxiv; Endothelial Cells are Heterogeneous in Different Brain Regions and are Dramatically Altered in Alzheimer's Disease; Authors - Annie Bryant
human	IF	J Neurosci; Endothelial Cells Are Heterogeneous in Different Brain Regions and Are Dramatically Altered in Alzheimer's Disease; Authors - Annie Bryant
rat	IF	Dev Cell; Nuclear import carrier Hikeshi cooperates with HSP70 to promote murine oligodendrocyte differentiation and CNS myelination; Authors - Li Li