HSPA1L Monoclonal antibody, PBS Only

HSPA1L Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3E7H2

Cat no : 66780-1-PBS

Synonyms

Heat shock 70 kDa protein 1L, HSP70 1L, HSP70 HOM, HSP70T, HSPA1L, hum70t

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Validation Data Gallery

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  WB analysis of HeLa using 66780-1-Ig (same clone as 66780-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66780-1-Ig (HSPA1L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66780-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 66780-1-Ig (same clone as 66780-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66780-1-Ig (HSPA1L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66780-1-PBS in a different storage buffer formulation.

• 

  WB analysis of L02 using 66780-1-Ig (same clone as 66780-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 66780-1-Ig (HSPA1L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66780-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 66780-1-Ig (same clone as 66780-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66780-1-Ig (HSPA1L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66780-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HSC-T6 using 66780-1-Ig (same clone as 66780-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66780-1-Ig (HSPA1L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66780-1-PBS in a different storage buffer formulation.

• 

  WB analysis of NIH/3T3 using 66780-1-Ig (same clone as 66780-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66780-1-Ig (HSPA1L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66780-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 66780-1-Ig (same clone as 66780-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66780-1-Ig (HSPA1L antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66780-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 66780-1-Ig (same clone as 66780-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66780-1-Ig (HSPA1L antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66780-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 66780-1-Ig (same clone as 66780-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 66780-1-Ig (HSPA1L antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66780-1-PBS in a different storage buffer formulation.

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Product Information

66780-1-PBS targets HSPA1L in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: HSPA1L fusion protein Ag5182
• Full Name: heat shock 70kDa protein 1-like
• Calculated Molecular Weight: 70 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC034483
• Gene Symbol: HSPA1L
• Gene ID (NCBI): 3305
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

HSPA1L (Hsp70-like protein 1, also known as HSP70-hom) is a member of Hsp70 family of heat shock proteins. Recently it has been reported that HSPA1L can interact with TLR4, activate dendritic cells and induce Th1 Response. This antibody well recognizes the endogenous HSPA1L protein in multiple cell lines. (21730052)