HSD17B12 Polyclonal antibody

HSD17B12 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 31463-1-AP

Synonyms

EC:1.1.1.330, 3-ketoacyl-CoA reductase, 3 ketoacyl CoA reductase, 17-beta-hydroxysteroid dehydrogenase 12, 17-beta-HSD 12

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Validation Data Gallery

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  WB analysis using 31463-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31463-1-AP (HSD17B12 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 31463-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 31463-1-AP (HSD17B12 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 31463-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 31463-1-AP (HSD17B12 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HT-29 cells, HuH-7 cells, MDA-MB-231 cells
• Positive IHC detected in: human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31463-1-AP targets HSD17B12 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HSD17B12 fusion protein Ag34833
• Full Name: hydroxysteroid (17-beta) dehydrogenase 12
• Calculated Molecular Weight: 312 aa, 34 kDa
• Observed Molecular Weight: ~34 kDa
• GenBank Accession Number: BC012043
• Gene Symbol: HSD17B12
• Gene ID (NCBI): 51144
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HSD17B12 (17β-hydroxysteroid dehydrogenase 12) is an important protein belonging to the hydroxysteroid dehydrogenase family, which is mainly involved in the metabolism of steroids and fatty acids in the body. It has diverse functions in cells and is involved in various biochemical reactions, especially playing a key role in sex hormone synthesis and fatty acid elongation. HSD17B12 can convert estrone into estradiol in ovarian tissue. This enzyme is also involved in fatty acid elongation. HSD17B12 is expressed in a variety of tissues, especially in the brain tissue and the digestive system. In disease states, such as colorectal cancer (CRC), the expression level of HSD17B12 is significantly increased, suggesting that it may play a role in the occurrence and development of tumors.