HSD17B1 Polyclonal antibody, PBS Only

HSD17B1 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 25334-1-PBS

Synonyms

20 alpha-hydroxysteroid dehydrogenase, 20 alpha HSD, 17β-HSD, 17-beta-hydroxysteroid dehydrogenase type 1, 17-beta-HSD 1

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Validation Data Gallery

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  WB analysis of human placenta using 25334-1-AP (same clone as 25334-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 25334-1-AP (HSD17B1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25334-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human placenta using 25334-1-AP (same clone as 25334-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 25334-1-AP (HSD17B1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25334-1-PBS in a different storage buffer formulation.

• 

  IP experiment of human placenta using 25334-1-AP (same clone as 25334-1-PBS)

  IP result of anti-HSD17B1 (IP:25334-1-AP, 4ug; Detection:25334-1-AP 1:500) with human placenta tissue lysate 2600 ug. This data was developed using the same antibody clone with 25334-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 25334-1-AP (same clone as 25334-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 25334-1-AP (HSD17B1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25334-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 25334-1-AP (same clone as 25334-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 25334-1-AP (HSD17B1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25334-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 25334-1-AP (same clone as 25334-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25334-1-AP (HSD17B1 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25334-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 25334-1-AP (same clone as 25334-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25334-1-AP (HSD17B1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25334-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 25334-1-AP (same clone as 25334-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25334-1-AP (HSD17B1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25334-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human endometrial cancer using 25334-1-AP (same clone as 25334-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 25334-1-AP (HSD17B1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25334-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human endometrial cancer using 25334-1-AP (same clone as 25334-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 25334-1-AP (HSD17B1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25334-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 25334-1-AP (same clone as 25334-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25334-1-AP (HSD17B1 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 25334-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 25334-1-AP (same clone as 25334-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25334-1-AP (HSD17B1 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 25334-1-PBS in a different storage buffer formulation.

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Product Information

25334-1-PBS targets HSD17B1 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HSD17B1 fusion protein Ag17886
• Full Name: hydroxysteroid (17-beta) dehydrogenase 1
• Calculated Molecular Weight: 328 aa, 35 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC104752
• Gene Symbol: HSD17B1
• Gene ID (NCBI): 3292
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.