HOXC8 Polyclonal antibody

• Featured Product
• KD/KO Validated

HOXC8 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 15448-1-AP

Synonyms

homeobox C8, Homeobox protein Hox 3A, Homeobox protein Hox C8, HOX3, HOX3A, HOXC8

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Validation Data Gallery

• 

  WB analysis of COLO 320 using 15448-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 15448-1-AP (HOXC8 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 15448-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 15448-1-AP (HOXC8 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MDA-MB-453s using 15448-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 15448-1-AP (HOXC8 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 15448-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 15448-1-AP (HOXC8 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: COLO 320 cells, MDA-MB-453s cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 6 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Product Information

15448-1-AP targets HOXC8 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, sheep
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HOXC8 fusion protein Ag7675
• Full Name: homeobox C8
• Calculated Molecular Weight: 27 aa, 7 kDa
• Observed Molecular Weight: 33-38 kDa
• GenBank Accession Number: BC053898
• Gene Symbol: HOXC8
• Gene ID (NCBI): 3224
• RRID: AB_2878140
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	IHC	Nature; Divergent transcriptional regulation of astrocyte reactivity across disorders.; Authors - Joshua E Burda
human	WB,CoIP	iScience; DLX2 promotes osteosarcoma epithelial-mesenchymal transition and doxorubicin resistance by enhancing HOXC8-CDH2 axis; Authors - Boya Zhang
human	WB	Oncogenesis; HOXC8 promotes proliferation and migration through transcriptional up-regulation of TGFβ1 in non-small cell lung cancer.; Authors - Houli Liu ; KD Validated
mouse	IF	iScience; Suv4-20h2 protects against influenza virus infection by suppression of chromatin loop formation.; Authors - Masami Shiimori
human	WB	Mol Carcinog; Upregulation of MGP by HOXC8 promotes the proliferation, migration, and EMT processes of triple-negative breast cancer.; Authors - Chen Gong ; KD Validated
human	WB	Int J Biochem Cell Biol; Homeobox C8 is a transcriptional repressor of E-cadherin gene expression in non-small cell lung cancer.; Authors - Jie Zhang ; KD Validated