HOXC13 Polyclonal antibody

HOXC13 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 27002-1-AP

Synonyms

HOX3G

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Validation Data Gallery

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  WB analysis using 27002-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27002-1-AP (HOXC13 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 27002-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HOXC13 antibody (27002-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Tested Applications

• Positive WB detected in: HeLa cells, NCCIT cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27002-1-AP targets HOXC13 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HOXC13 fusion protein Ag25544
• Full Name: homeobox C13
• Calculated Molecular Weight: 35 kDa, 330 aa
• Observed Molecular Weight: 38 kDa
• GenBank Accession Number: BC090850
• Gene Symbol: HOXC13
• Gene ID (NCBI): 3229
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HOXC13 is a member of HOX proteins. The HOX family includes 39 paralogous proteins expressed from the HOXA, HOXB, HOXC, and HOXD gene clusters, and these function early in embryonic development to establish cell and tissue identity and to regulate cell proliferation, differentiation, and survival (PMID: 30974835). Human HOXC13, which is expressed in the hair follicle and nail matrix, is involved in the regulation of keratin gene expression (PMID: 11714694). Mutations in human HOXC13 cause ectodermal dysplasia 9, a severe defect of hair and nails (PMID: 23063621).