HOXB7 Polyclonal antibody

HOXB7 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 12613-1-AP

Synonyms

HOX2C, Homeobox protein Hox-B7, Homeobox protein Hox-2C, Homeobox protein HHO.C1, HHO.C1

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Validation Data Gallery

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  WB analysis of SKOV-3 using 12613-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 12613-1-AP (HOXB7 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SKOV-3 using 12613-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 12613-1-AP (HOXB7 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

• 

  IP experiment of SKOV-3 using 12613-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 12613-1-AP (HOXB7 antibody) at dilution of 1:300.

• 

  IF Staining of HepG2 using 12613-1-AP

  Immunofluorescent analysis of HepG2 cells using 12613-1-AP (HOXB7 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: SKOV-3 cells
• Positive IP detected in: SKOV-3 cells
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IHC: See 1 publications below

Product Information

12613-1-AP targets HOXB7 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HOXB7 fusion protein Ag3245
• Full Name: homeobox B7
• Calculated Molecular Weight: 217 aa, 24 kDa
• Observed Molecular Weight: 24-30 kDa
• GenBank Accession Number: BC015345
• Gene Symbol: HOXB7
• Gene ID (NCBI): 3217
• RRID: AB_2877867
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
rat	WB	J Neuroinflammation; Gene expression comparison reveals distinct basal expression of HOX members and differential TNF-induced response between brain- and spinal cord-derived microvascular endothelial cells.; Authors - Yves Molino
human	WB	Int J Oncol; CircRNA hsa_circ_0070934 functions as a competitive endogenous RNA to regulate HOXB7 expression by sponging miR‑1236‑3p in cutaneous squamous cell carcinoma.; Authors - Da-Wei Zhang
human	WB	Exp Cell Res; Long non-coding RNA BBOX1-AS1 exacerbates esophageal squamous cell carcinoma development by regulating HOXB7/β-catenin axis.; Authors - Jinxiu Sheng
human	WB,IHC	Biomed Res Int; Cell Cycle-Related Gene SPC24: A Novel Potential Diagnostic and Prognostic Biomarker for Laryngeal Squamous Cell Cancer; Authors - Jialei Chen