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  • KD/KO Validated

HOOK3 Polyclonal antibody

HOOK3 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 15457-1-AP

Synonyms

h hook3, hHK3, HK3, hook homolog 3 (Drosophila), HOOK3, Protein Hook homolog 3



Tested Applications

Positive WB detected inrat liver tissue, HEK-293 cells, mouse kidney tissue, human liver tissue, mouse heart tissue, mouse liver tissue
Positive IP detected inmouse kidney tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

15457-1-AP targets HOOK3 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HOOK3 fusion protein Ag7724
Full Name hook homolog 3 (Drosophila)
Calculated molecular weight 83 kDa
Observed molecular weight 83 kDa
GenBank accession numberBC056146
Gene symbol HOOK3
Gene ID (NCBI) 84376
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for HOOK3 antibody 15457-1-APDownload protocol
IHC protocol for HOOK3 antibody 15457-1-APDownload protocol
IF protocol for HOOK3 antibody 15457-1-APDownload protocol
IP protocol for HOOK3 antibody 15457-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

PTPN21 and Hook3 relieve KIF1C autoinhibition and activate intracellular transport.

Authors - Nida Siddiqui
ratWB

Dev Cell

The Dynein Adaptor RILP Controls Neuronal Autophagosome Biogenesis, Transport, and Clearance.

Authors - Noopur V Khobrekar
humanWB,IP

J Cell Biol

Hook3 is a scaffold for the opposite-polarity microtubule-based motors cytoplasmic dynein-1 and KIF1C.

Authors - Agnieszka A Kendrick
ratWB

J Cell Biol

Dynein activator Hook1 is required for trafficking of BDNF-signaling endosomes in neurons.

Authors - Mara A Olenick
mouseWB

Mol Cell Proteomics

Spatiotemporal Changes of the Phagosomal Proteome in Dendritic Cells in Response to LPS Stimulation

Authors - Anne-Marie Pauwels
humanWB

iScience

Mitotic HOOK3 phosphorylation by ERK1c drives microtubule-dependent Golgi destabilization and fragmentation.

Authors - Inbal Wortzel
  • KO Validated