HOOK3 Polyclonal antibody

• Featured Product
• KD/KO Validated

HOOK3 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

12

Cat no : 15457-1-AP

Synonyms

h hook3, hHK3, HK3, hook homolog 3 (Drosophila), HOOK3, Protein Hook homolog 3

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Validation Data Gallery

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  WB analysis of rat liver using 15457-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 15457-1-AP (HOOK3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 15457-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 15457-1-AP (HOOK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 15457-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15457-1-AP (HOOK3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 15457-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15457-1-AP (HOOK3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 15457-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15457-1-AP (HOOK3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 15457-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 15457-1-AP (HOOK3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 15457-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 15457-1-AP (HOOK3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse kidney using 15457-1-AP

  IP result of anti-HOOK3 (IP:15457-1-AP, 4ug; Detection:15457-1-AP 1:500) with mouse kidney tissue lysate 4000ug.

• 

  IHC staining of human kidney using 15457-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15457-1-AP (HOOK3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 15457-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15457-1-AP (HOOK3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 15457-1-AP

  Immunofluorescent analysis of HepG2 cells, using HOOK3 antibody 15457-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: rat liver tissue, HEK-293 cells, mouse kidney tissue, human liver tissue, mouse heart tissue, mouse liver tissue
• Positive IP detected in: mouse kidney tissue
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 11 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

15457-1-AP targets HOOK3 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HOOK3 fusion protein Ag7724
• Full Name: hook homolog 3 (Drosophila)
• Calculated Molecular Weight: 83 kDa
• Observed Molecular Weight: 83 kDa
• GenBank Accession Number: BC056146
• Gene Symbol: HOOK3
• Gene ID (NCBI): 84376
• RRID: AB_2120828
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Nat Commun; PTPN21 and Hook3 relieve KIF1C autoinhibition and activate intracellular transport.; Authors - Nida Siddiqui
rat	WB	Dev Cell; The Dynein Adaptor RILP Controls Neuronal Autophagosome Biogenesis, Transport, and Clearance.; Authors - Noopur V Khobrekar
human	WB,IP	J Cell Biol; Hook3 is a scaffold for the opposite-polarity microtubule-based motors cytoplasmic dynein-1 and KIF1C.; Authors - Agnieszka A Kendrick
rat	WB	J Cell Biol; Dynein activator Hook1 is required for trafficking of BDNF-signaling endosomes in neurons.; Authors - Mara A Olenick
mouse	WB	Mol Cell Proteomics; Spatiotemporal Changes of the Phagosomal Proteome in Dendritic Cells in Response to LPS Stimulation; Authors - Anne-Marie Pauwels
human	WB	iScience; Mitotic HOOK3 phosphorylation by ERK1c drives microtubule-dependent Golgi destabilization and fragmentation.; Authors - Inbal Wortzel ; KO Validated