HOOK2 Polyclonal antibody

HOOK2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 12458-1-AP

Synonyms

Hook Microtubule Tethering Protein 2, hook homolog 2 (Drosophila), HK2, h-hook2, hHK2

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Validation Data Gallery

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  WB analysis using 12458-1-AP

  SH-SY5Y cell lysates were subjected to SDS PAGE followed by western blot with 12458-1-AP (HOOK2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse colon using 12458-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 12458-1-AP (HOOK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 12458-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using HOOK2 antibody (12458-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Tested Applications

• Positive WB detected in: SH-SY5Y cells
• Positive IHC detected in: mouse colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12458-1-AP targets HOOK2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HOOK2 fusion protein Ag3124
• Full Name: hook homolog 2 (Drosophila)
• Calculated Molecular Weight: 717 aa, 83 kDa
• Observed Molecular Weight: 83 kDa
• GenBank Accession Number: BC012443
• Gene Symbol: HOOK2
• Gene ID (NCBI): 29911
• RRID: AB_3669139
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.