HOGA1 Polyclonal antibody

HOGA1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 27755-1-AP

Synonyms

C10orf65, DHDPS like protein, DHDPSL, HOGA1, Protein 569272

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Validation Data Gallery

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  WB analysis using 27755-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27755-1-AP (HOGA1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse kidney tissue, rat kidney tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27755-1-AP targets HOGA1 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HOGA1 fusion protein Ag26849
• Full Name: chromosome 10 open reading frame 65
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC045550
• Gene Symbol: HOGA1
• Gene ID (NCBI): 112817
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HOGA1 (4-hydroxy-2-oxoglutarate aldolase), also known as C10orf65, DHDPSL, is a mitochondrial enzyme that plays a gatekeeper role in hydroxyproline metabolis. HOGA1 is primarily expressed in liver and kidney and catalyzes the final step of mitochondrial hydroxyproline metabolism\ from 4-hydroxy-2-oxoglutarate to glyoxylate and pyruvate (PMID: 31696211). Identification of mutations in the HOGA1 gene as the cause of autosomal recessive primary hyperoxaluria (PH) type III has revitalized research in the field of PH and related stone disease (PMID: 22781098).