HNRNPH3 Polyclonal antibody

HNRNPH3 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 17674-1-AP

Synonyms

HNRPH3, hnRNP H3, hnRNP 2H9

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Validation Data Gallery

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  WB analysis using 17674-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17674-1-AP (HNRNPH3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IF Staining of PC-3 using 17674-1-AP

  Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using HNRNPH3 antibody (17674-1-AP) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, K-562 cells, PC-3 cells, mouse testis tissue
• Positive IF/ICC detected in: PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below

Product Information

17674-1-AP targets HNRNPH3 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HNRNPH3 fusion protein Ag11611
• Full Name: heterogeneous nuclear ribonucleoprotein H3 (2H9)
• Calculated Molecular Weight: 331 aa, 35 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC039824
• Gene Symbol: HNRNPH3
• Gene ID (NCBI): 3189
• RRID: AB_2878425
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB,IF	Dis Model Mech; Loss of Ranbp2 in motor neurons causes the disruption of nucleocytoplasmic and chemokine signaling and proteostasis of hnRNPH3 and Mmp28, and the development of amyotrophic lateral sclerosis (ALS)-like syndromes.; Authors - Kyoung-In Cho