HNRNPA2B1 Polyclonal antibody

• Featured Product
• KD/KO Validated

HNRNPA2B1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, RIP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

23

Cat no : 14813-1-AP

Synonyms

hnRNP A2/B1, HNRNPA2, HNRNPA2B1, HNRNPB1, HNRPA2, HNRPA2B1, HNRPB1, RNPA2, SNRPB1

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Validation Data Gallery

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  WB analysis using 14813-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14813-1-AP (HNRNPA2B1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 14813-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14813-1-AP (HNRNPA2B1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis using 14813-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14813-1-AP (HNRNPA2B1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 14813-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 14813-1-AP (HNRNPA2B1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human lymphoma using 14813-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 14813-1-AP (HNRNPA2B1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human cervical cancer using 14813-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14813-1-AP (HNRNPA2B1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 14813-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14813-1-AP (HNRNPA2B1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 14813-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14813-1-AP (HNRNPA2B1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 14813-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14813-1-AP (HNRNPA2B1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 14813-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 14813-1-AP (HNRNPA2B1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of HeLa using 14813-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 14813-1-AP (HNRNPA2B1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A431 cells, A549 cells, Jurkat cells, Raji cells, HL-60 cells, HSC-T6 cells, HeLa cells, K-562 cells
• Positive IHC detected in: human lymphoma tissue, human colon cancer tissue, human cervical cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 5 publications below
• WB: See 17 publications below
• IHC: See 5 publications below
• IF: See 3 publications below
• RIP: See 2 publications below

Product Information

14813-1-AP targets HNRNPA2B1 in WB, RIP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HNRNPA2B1 fusion protein Ag6563
• Full Name: heterogeneous nuclear ribonucleoprotein A2/B1
• Calculated molecular weight: 37 kDa
• Observed molecular weight: 36 kDa and 38 kDa
• GenBank accession number: BC000506
• Gene symbol: HNRNPA2B1
• Gene ID (NCBI): 3181
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

HnRNPA2B1 in one of heterogenous nuclear ribonucleoproteins (HnRNPs), which regulate the splicing and transportation of mRNA. It has a role in packaging nascent mRNA, alternative splicing, tycoplamic RNA trafficking, translation and stabilization. Besides it may involve in telomere maintenance, cell proliferation and differentiation, and glucose transport. Gene HURNPA2B1 encode two protein, HNRNPA2 (38kd) and HNRNPB1 (36kd), by alternative splicing.

Publications

Species	Application	Title
human	IHC	Med Sci Monit; Expression and Prognostic Significance of m6A-Related Genes in Lung Adenocarcinoma.; Authors - Yingying Zhang
mouse	WB	bioRxiv; Novel gene-specific translation mechanism of dysregulated, chronic inflammation reveals promising, multifaceted COVID-19 therapeutics.; Authors - Li Wang
mouse	WB	Nat Struct Mol Biol; TDP-43 aggregation induced by oxidative stress causes global mitochondrial imbalance in ALS.; Authors - Xinxin Zuo
human	WB	Int J Mol Sci; hnRNPA2B1-Mediated Extracellular Vesicles Sorting of miR-122-5p Potentially Promotes Lung Cancer Progression.; Authors - Chuang Li
human	IHC	Technol Cancer Res Treat; Four m6A RNA Methylation Gene Signatures and Their Prognostic Values in Lung Adenocarcinoma.; Authors - Yuzhu Wang
human	WB	Int J Mol Sci; Kaempferol Can Reverse the 5-Fu Resistance of Colorectal Cancer Cells by Inhibiting PKM2-Mediated Glycolysis.; Authors - Haili Wu