HMMR-Specific Polyclonal antibody

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HMMR-Specific Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Cat No. 15820-1-AP Copied!

Publications (25)

Reviews (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Synonyms

HMMR, CD168, IHABP, Intracellular hyaluronic acid-binding protein, Receptor for hyaluronan-mediated motility

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  WB analysis using 15820-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15820-1-AP (HMMR-Specific antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HepG2 using 15820-1-AP

  WB result of HMMR-Specific antibody (15820-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HMMR-Specific transfected HepG2 cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human tonsillitis using 15820-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15820-1-AP (HMMR-Specific Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human tonsillitis using 15820-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15820-1-AP (HMMR-Specific Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HepG2 using 15820-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using HMMR-Specific antibody (15820-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of HepG2 using 15820-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human HMMR-Specific (15820-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HepG2 cells, K-562 cells, C6 cells, T-47D cells
• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15820-1-AP targets HMMR-Specific in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: hyaluronan-mediated motility receptor (RHAMM)
• Calculated Molecular Weight: 84 kDa
• Observed Molecular Weight: 84 kDa
• GenBank Accession Number: NM_001142556
• Gene Symbol: HMMR
• Gene ID (NCBI): 3161
• RRID: AB_2295342
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O75330
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Hyaluronan-mediated motility receptor (HMMR), also termed CD168, was first described by Turley in murine cells . It is reported that HMMR has an extensive coiled-coil structure (CC) that contains multiple sites for interactive partners. Initially, HMMR was considered a novel hyaluronan-mediated motility receptor and a microtubule-associated spindle assembly factor. Full-length human RHAMM is an 85  kDa coiled-coil protein that occurs both in intracellular and extracellular compartments. It has highly restricted and tightly regulated expression in most normal tissues, but is one of a number of oncogenic proteins that are exported to the cell surface in response to tissue stress by unconventional transport mechanisms. (PMID: 36750558, PMID: 30249497)

Publications

What published studies show

Anti-HMMR (Hyaluronan Mediated Motility Receptor) antibody, catalog number 15820-1-AP, has 25 citations across 22 journals, including Nature, Science, Int J Biol Sci, Cell Death Discov, Cell Signal, and FEBS Lett. Research fields span cancer biology (prostate, lung, colon, liver, osteosarcoma, head and neck), stem cell biology, oocyte biology, atherosclerosis, and muscle atrophy.

Automatically generated from the 25 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse		Nature; Tracing haematopoietic stem cell formation at single-cell resolution.; Authors - Fan Zhou
human	IF	Science; The mechanism of acentrosomal spindle assembly in human oocytes; Authors - Tianyu Wu
		Sci Bull (Beijing); The mechanisms of MTOCs maturation in human and mouse oocytes.; Authors - Hao Gu
human	WB	Int J Biol Sci; Oleate acid-stimulated HMMR expression by CEBPα is associated with nonalcoholic steatohepatitis and hepatocellular carcinoma.; Authors - Deyu Zhang ; KD Validated
human	WB,CoIP	Int J Biol Sci; N6-methyladenosine Reader IGF2BP2-modified HMMR Promotes Non-small Cell Lung Cancer Metastasis via Interaction with MAP4K4; Authors - Jiansheng Zhang ; KD Validated
human	WB,IP,IHC,IF	Cell Death Discov; HMMR promotes prostate cancer proliferation and metastasis via AURKA/mTORC2/E2F1 positive feedback loop; Authors - Kaixuan Guo ; KD Validated

Reviews

2.0 out of 5

1 verified customer reviews

What customers say

One reviewer tested this antibody by Western Blot on renal carcinoma cell lines (SN12L1, SN12C, ACHN) at dilutions of 1:500–1:1000 and reported multiple non-specific bands at various molecular weights, finding it unsuitable for specific HMMR detection. The review was rated 2 out of 5 stars.

Automatically generated from the 1 verified customer reviews below by Able AI.

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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Heriberto (Verified Customer) (11-28-2018)

Observed multiple bands at different molecular weights. Was not specific for the detection of HMMR .

• Applications: Western Blot,
• Primary Antibody Dilution: Try 1:1000, 1:750 and 1:500
• Cell Tissue Type: SN12L1, SN12C and ACHN- Renal carcinoma cell lines

HMMR-Specific Antibody Western Blot, validation (Try 1:1000, 1:750 and 1:500 dilution) in SN12L1, SN12C and ACHN- Renal carcinoma cell lines (Cat no:15820-1-AP)