HMMR-Specific Polyclonal antibody

• Featured Product
• KD/KO Validated

HMMR-Specific Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

10

Reviews (1)

Cat no : 15820-1-AP

Synonyms

CD168, HMMR, HMMR-Specific, IHABP, RHAMM

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Validation Data Gallery

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  WB analysis using 15820-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15820-1-AP (HMMR-Specific antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 15820-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15820-1-AP (HMMR-Specific Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 15820-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15820-1-AP (HMMR-Specific Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 15820-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using HMMR-Specific antibody (15820-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HepG2 using 15820-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human HMMR-Specific (15820-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: HepG2 cells, K-562 cells, C6 cells, T-47D cells
• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells
• Positive FC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 5 publications below
• IP: See 1 publications below
• FC: See 2 publications below

Product Information

15820-1-AP targets HMMR-Specific in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: hyaluronan-mediated motility receptor (RHAMM)
• Calculated molecular weight: 84 kDa
• Observed molecular weight: 84 kDa
• GenBank accession number: NM_001142556
• Gene symbol: HMMR
• Gene ID (NCBI): 3161
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HMMR, also named RHAMM, is a hyaluronan receptor expressed in various tissues. It is involved in regulation of focal contacts and subsequent motility, thus playing roles in wound healing and tumor metastasis. Remarkably different molecular masses of RHAMM have been published. Antibodies raised against RHAMM have been reported to recognize proteins of 56, 66 and 70 kDa in the supernatant of murine 3T3 fibroblasts(PMID: 2440472). Some of the isoforms detectable by anti-RHAMM antibodies, including the 52 kDa form and a 48-49 kDa form found in 10T1/2 cells, have been speculated to be underglycosylated precursor forms of the mature RHAMM protein(PMID: 1705559).

Publications

Species	Application	Title
mouse	FC	Nature; Tracing haematopoietic stem cell formation at single-cell resolution.; Authors - Fan Zhou
rat	IF	Int J Mol Sci; Effects of Therapeutic Platelet-Rich Plasma on Overactive Bladder via Modulating Hyaluronan Synthesis in Ovariectomized Rat; Authors - Jian-He Lu
human	WB	Int J Biol Sci; Oleate acid-stimulated HMMR expression by CEBPα is associated with nonalcoholic steatohepatitis and hepatocellular carcinoma.; Authors - Deyu Zhang ; KD Validated
rat	WB	J Cell Physiol; A pH-sensitive luminal His-cluster promotes interaction of PAM with V-ATPase along the secretory and endocytic pathways of peptidergic cells.; Authors - Vishwanatha K Rao
human	WB	Cell Signal; Fam83d modulates MAP kinase and AKT signaling and is induced during neurogenic skeletal muscle atrophy.; Authors - Lisa M Cooper
human	IHC	J Thorac Dis; Development and validation of a combined metabolism and immune prognostic model in lung adenocarcinoma; Authors - Yu Shi

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Heriberto (Verified Customer) (11-28-2018)

Observed multiple bands at different molecular weights. Was not specific for the detection of HMMR .

• Applications: Western Blot,
• Primary Antibody Dilution: Try 1:1000, 1:750 and 1:500
• Cell Tissue Type: SN12L1, SN12C and ACHN- Renal carcinoma cell lines

HMMR-Specific Antibody Western Blot, validation (Try 1:1000, 1:750 and 1:500 dilution) in SN12L1, SN12C and ACHN- Renal carcinoma cell lines (Cat no:15820-1-AP)

Related Products

Flow Cytometry Perm Buffer (10X)

PF00011-C