HMHA1 Polyclonal antibody
HMHA1 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IF, IHC, ELISA
Conjugate
Unconjugated
Cat no : 14832-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | A375 cells, K-562 cells, mouse thymus tissue, HeLa cells, Jurkat cells |
Positive IHC detected in | human tonsil tissue, human spleen tissue, mouse spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:8000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
IF | See 1 publications below |
Product Information
14832-1-AP targets HMHA1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | HMHA1 fusion protein Ag6636 |
Full Name | histocompatibility (minor) HA-1 |
Calculated Molecular Weight | 1136 aa, 125 kDa |
Observed Molecular Weight | 140-150 kDa |
GenBank Accession Number | BC065223 |
Gene Symbol | HMHA1 |
Gene ID (NCBI) | 23526 |
RRID | AB_10642145 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
Product Specific Protocols | |
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WB protocol for HMHA1 antibody 14832-1-AP | Download protocol |
IHC protocol for HMHA1 antibody 14832-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Aging Dis Microglia Exhibit Distinct Heterogeneity Rather than M1/M2 Polarization within the Early Stage of Acute Ischemic Stroke |