HeLa cells were subjected to SDS PAGE followed by western blot with 14582-1-AP (HMG20B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
HeLa cells were subjected to SDS PAGE followed by western blot with 14582-1-AP (HMG20B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 14582-1-AP
WB result of HMG20B antibody (14582-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HMG20B transfected HeLa cells.
Immunohistochemical analysis of paraffin-embedded human colon cancer using 14582-1-AP (HMG20B antibody) at dilution of 1:50 (under 40x lens).
IF Staining of HeLa using 14582-1-AP
Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 14582-1-AP (HMG20B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 14582-1-AP (HMG20B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
HeLa cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:500-1:1000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:20-1:200
Immunofluorescence (IF)/ICC
IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
CoREST (corepressor of repressor element-1-silencing transcription factor) is a chromatin- modifying complex that was first identified as a regulator of neuronal gene expression, having a essential role in neuronal differentiation [PMID:10449787]. The CoREST complex is recruited by REST (RE1-silencing transcription factor) that recognizes the 21-nucleotide RE-1 sequence in the promoter of more than 1,700 different target genes in human and mouse [PMID:15240883]. HMGB20B is one component of the CoREST complex, which mediates chromatin remodeling and repression of specific genes involved in neuronal differentiation through modulation of chromatin structure [PMID:19997604].
HeLa cells were subjected to SDS PAGE followed by western blot with 14582-1-AP (HMG20B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 14582-1-AP
WB result of HMG20B antibody (14582-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HMG20B transfected HeLa cells.
IHC Figures
IHC staining of human colon cancer using 14582-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer using 14582-1-AP (HMG20B antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human colon cancer using 14582-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer using 14582-1-AP (HMG20B antibody) at dilution of 1:50 (under 40x lens).
IP Figures
IP experiment of HeLa using 14582-1-AP
IP result of anti-HMG20B (IP:14582-1-AP, 4ug; Detection:14582-1-AP 1:800) with HeLa cells lysate 1200ug.
IF/ICC Figures
IF Staining of HeLa using 14582-1-AP
Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 14582-1-AP (HMG20B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
KD/KO VALIDATED
HMG20B Polyclonal antibody
Catalog Number
14582-1-AP
Citations
4
Dilutions
WB : 1:500-1:1000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:20-1:200 IF/ICC : 1:50-1:500
Applications
WB, IHC, IF/ICC, IP, ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
