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HMG20B Polyclonal antibody

HMG20B Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 14582-1-AP


BRAF25, BRAF35, BRCA2 associated factor 35, high mobility group 20B, HMG box containing protein 20B, HMG20B, HMGX2, HMGXB2, PP7706, pp8857, SMARCE1 related protein, SMARCE1r, SOXL

Tested Applications

Positive WB detected inHeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

14582-1-AP targets HMG20B in WB, IP, IHC, IF, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HMG20B fusion protein Ag6103
Full Name high-mobility group 20B
Calculated molecular weight 36 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC002552
Gene symbol HMG20B
Gene ID (NCBI) 10362
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CoREST (corepressor of repressor element-1-silencing transcription factor) is a chromatin- modifying complex that was first identified as a regulator of neuronal gene expression, having a essential role in neuronal differentiation [PMID:10449787]. The CoREST complex is recruited by REST (RE1-silencing transcription factor) that recognizes the 21-nucleotide RE-1 sequence in the promoter of more than 1,700 different target genes in human and mouse [PMID:15240883]. HMGB20B is one component of the CoREST complex, which mediates chromatin remodeling and repression of specific genes involved in neuronal differentiation through modulation of chromatin structure [PMID:19997604].


Product Specific Protocols
WB protocol for HMG20B antibody 14582-1-APDownload protocol
IHC protocol for HMG20B antibody 14582-1-APDownload protocol
IF protocol for HMG20B antibody 14582-1-APDownload protocol
IP protocol for HMG20B antibody 14582-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Nat Commun

The H2A.Z and NuRD associated protein HMG20A controls early head and heart developmental transcription programs

Authors - Andreas Herchenröther

Sci Adv

Targeting the scaffolding role of LSD1 (KDM1A) poises acute myeloid leukemia cells for retinoic acid-induced differentiation.

Authors - Roberto Ravasio

EMBO Mol Med

LSD1 inhibition induces differentiation and cell death in Merkel cell carcinoma.

Authors - Lukas Leiendecker


The DNA binding factor Hmg20b is a repressor of erythroid differentiation.

Authors - Esteghamat Fatemehsadat F