HLA-DRB5 Polyclonal antibody, PBS Only

HLA-DRB5 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 21702-1-PBS

Synonyms

HLA DRB, Dw2, DR2-beta-2, DR beta-5, DR beta 5

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Validation Data Gallery

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  WB analysis of HepG2 using 21702-1-AP (same clone as 21702-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 21702-1-AP (HLA-DRB5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21702-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 21702-1-AP (same clone as 21702-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 21702-1-AP (HLA-DRB5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21702-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HL-60 using 21702-1-AP (same clone as 21702-1-PBS)

  HL-60 cells were subjected to SDS PAGE followed by western blot with 21702-1-AP (HLA-DRB5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21702-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Raji using 21702-1-AP (same clone as 21702-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 21702-1-AP (HLA-DRB5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21702-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 21702-1-AP (same clone as 21702-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21702-1-AP (HLA-DRB5 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21702-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 21702-1-AP (same clone as 21702-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21702-1-AP (HLA-DRB5 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21702-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 21702-1-AP (same clone as 21702-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21702-1-AP (HLA-DRB5 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21702-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 21702-1-AP (same clone as 21702-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21702-1-AP (HLA-DRB5 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21702-1-PBS in a different storage buffer formulation.

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Product Information

21702-1-PBS targets HLA-DRB5 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HLA-DRB5 fusion protein Ag16307
• Full Name: major histocompatibility complex, class II, DR beta 5
• Calculated Molecular Weight: 266 aa, 30 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC009234
• Gene Symbol: HLA-DRB5
• Gene ID (NCBI): 3127
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.