HLA-DRA Polyclonal antibody

HLA-DRA Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 17221-1-AP

Synonyms

MHC class II antigen DR, HLA-DRA1, HLA-DR, HLA DRB, HLA DRA1

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Validation Data Gallery

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  WB analysis using 17221-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17221-1-AP (HLA-DRA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat spleen using 17221-1-AP

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 17221-1-AP (HLA-DRA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of Raji using 17221-1-AP

  IP result of anti-HLA-DRA (IP:17221-1-AP, 4ug; Detection:17221-1-AP 1:1000) with Raji cells lysate 3200 ug.

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  IHC staining of human tonsillitis using 17221-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17221-1-AP (HLA-DRA Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human tonsillitis using 17221-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17221-1-AP (HLA-DRA Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human appendicitis using 17221-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 17221-1-AP (HLA-DRA antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human appendicitis using 17221-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 17221-1-AP (HLA-DRA antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of Raji using 17221-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Raji cells using HLA-DRA antibody (17221-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: Daudi cells, rat spleen tissue, Raji cells, Ramos cells
• Positive IP detected in: Raji cells
• Positive IHC detected in: human tonsillitis tissue, human appendicitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: Raji cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 4 publications below
• IF: See 3 publications below

Product Information

17221-1-AP targets HLA-DRA in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HLA-DRA fusion protein Ag11044
• Full Name: major histocompatibility complex, class II, DR alpha
• Calculated Molecular Weight: 254 aa, 29 kDa
• Observed Molecular Weight: 32-34 kDa
• GenBank Accession Number: BC032350
• Gene Symbol: HLA-DRA
• Gene ID (NCBI): 3122
• RRID: AB_10639510
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IF	Nat Commun; Single-cell RNA sequencing highlights the role of inflammatory cancer-associated fibroblasts in bladder urothelial carcinoma.; Authors - Zhaohui Chen
rat	IF	Aging Cell; Donor BMSC-derived small extracellular vesicles relieve acute rejection post-renal allograft through transmitting Loc108349490 to dendritic cells.; Authors - Zhi-Gang Wang
human	WB	Front Immunol; An Immunological Perspective: What Happened to Pregnant Women After Recovering From COVID-19?; Authors - Sijia Zhao
human	IF	Front Immunol; Functional Change of Effector Tumor-Infiltrating CCR5+CD38+HLA-DR+CD8+ T Cells in Glioma Microenvironment.; Authors - Pin-Yuan Chen
rat	IHC	Mol Med; Correction of immunosuppression in aged septic rats by human ghrelin and growth hormone through the vagus nerve-dependent inhibition of TGF-β production.; Authors - Mian Zhou
human	IHC	BMC Cancer; HLA class II molecule HLA-DRA identifies immuno-hot tumors and predicts the therapeutic response to anti-PD-1 immunotherapy in NSCLC.; Authors - Jie Mei