HK3 Polyclonal antibody

HK3 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 13333-1-AP

Synonyms

Hexokinase 3, hexokinase 3 (white cell), Hexokinase type III, HK III, HK3, HKIII, HXK3

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Validation Data Gallery

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  IHC staining of human lung cancer using 13333-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 13333-1-AP (HK3 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human lung cancer using 13333-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 13333-1-AP (HK3 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 5 publications below

Product Information

13333-1-AP targets HK3 in IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HK3 fusion protein Ag4148
• Full Name: hexokinase 3 (white cell)
• Calculated Molecular Weight: 923 aa, 99 kDa
• GenBank Accession Number: BC028129
• Gene Symbol: HK3
• Gene ID (NCBI): 3101
• RRID: AB_2877940
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IHC	Front Immunol; Novel macrophage-related gene prognostic index for glioblastoma associated with M2 macrophages and T cell dysfunction; Authors - Hang Ji
rat	IHC	Neurochem Int; Spatio-temporal expression of Hexokinase-3 in the injured female rat spinal cords.; Authors - Yu-Hong Lin
human	IHC	Front Immunol; High iodine promotes autoimmune thyroid disease by activating hexokinase 3 and inducing polarization of macrophages towards M1; Authors - Tiantian Cai
mouse		Sci Rep; Liver-specific overexpression of HKDC1 increases hepatocyte size and proliferative capacity; Authors - Carolina M Pusec
human		Biochem Pharmacol; Effects of propranolol on glucose metabolism in hemangioma-derived endothelial cells; Authors - Kaiying Yang
human,mouse		J Ethnopharmacol; Diosmetin-7-O-β-D-glucopyranoside from Pogostemonis Herba alleviated SARS-CoV-2-induced pneumonia by reshaping macrophage polarization and limiting viral replication; Authors - Yun-Lu Xu