Hexokinase 1 Polyclonal antibody

Hexokinase 1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 15656-1-AP

Synonyms

Brain form hexokinase, Cell and organelle markers, hexokinase 1, Hexokinase type I, HK I, HK1, HK1 ta, HK1 tb, HK1 tc, HKI, HXK1

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Validation Data Gallery

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  WB analysis using 15656-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15656-1-AP (Hexokinase 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: MCF-7 cells, U-87 MG cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 6 publications below
• IHC: See 1 publications below

Product Information

15656-1-AP targets Hexokinase 1 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Hexokinase 1 fusion protein Ag8008
• Full Name: hexokinase 1
• Calculated Molecular Weight: 102 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC008730
• Gene Symbol: HK1
• Gene ID (NCBI): 3098
• RRID: AB_2878162
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The hexokinases (HKs) catalyze the first obligatory step in glucose metabolism to generate glucose-6-phosphate. This not only furthers glucose entry by maintaining the concentration gradient for facilitated glucose influx but also provides the first intermediate for essentially all major pathways using glucose. There are four conventional HK isoforms, HK1/2/3/4, encoded by four different genes. Most adult tissues express only HK1. Muscle and adipose tissue use HK2 for glycolysis, liver, and pancreatic beta cells express HK4 (also called glucokinase) and do not express HK1 or HK2. (PMID: 31434645, PMID: 31848318)

Publications

Species	Application	Title
human	WB	Oncogene; HDAC6-G3BP2 promotes lysosomal-TSC2 and suppresses mTORC1 under ETV4 targeting-induced low-lactate stress in non-small cell lung cancer; Authors - Bei Liu
human	WB	J Cell Mol Med; Novel long non-coding RNA CYB561-5 promotes aerobic glycolysis and tumorigenesis by interacting with basigin in non-small cell lung cancer.; Authors - Longfei Li
human	WB	Cell Signal; BDNF corrects NLRP3 inflammasome-induced pyroptosis and glucose metabolism reprogramming through KLF2/HK1 pathway in vascular endothelial cells.; Authors - Hong Jin
rat	WB	Front Neurosci; Impaired Brain Energy Metabolism: Involvement in Depression and Hypothyroidism.; Authors - Katarzyna Głombik
human	WB	Onco Targets Ther; Prognostic Significance and Related Mechanisms of Hexokinase 1 in Ovarian Cancer.; Authors - Yanqing Li
	IHC	Med Sci Monit; PMID: 31332157; Authors - Li Yang