HIST1H2AC Polyclonal antibody

HIST1H2AC Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 15953-1-AP

Synonyms

H2AC6, H2A/l, Histone H2A, histone cluster 1, H2ac, H2AFL

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Validation Data Gallery

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  WB analysis of mouse liver using 15953-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15953-1-AP (HIST1H2AC Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of rat liver using 15953-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 15953-1-AP (HIST1H2AC Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human skin cancer using 15953-1-AP

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 15953-1-AP (HIST1H2AC antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse liver tissue, rat liver tissue
• Positive IHC detected in: human skin cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

15953-1-AP targets HIST1H2AC in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HIST1H2AC fusion protein Ag8663
• Full Name: histone cluster 1, H2ac
• Calculated Molecular Weight: 130 aa, 14 kDa
• Observed Molecular Weight: 17 kDa
• GenBank Accession Number: BC017379
• Gene Symbol: HIST1H2AC
• Gene ID (NCBI): 8334
• RRID: AB_2878199
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Biology (Basel); Identification of NRAS Downstream Genes with CRISPR Activation Screening; Authors - Akiya Tatsumi