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HINT1 Polyclonal antibody

HINT1 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 10717-1-AP


HINT, HINT1, PKCI 1, PKCI1, PRKCNH1, Protein kinase C inhibitor 1

Tested Applications

Positive WB detected inJurkat cells, rat brain tissue, rat thymus tissue, K-562 cells, Raji cells
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman colon cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Sample-dependent, check data in validation data gallery

Product Information

10717-1-AP targets HINT1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HINT1 fusion protein Ag1142
Full Name histidine triad nucleotide binding protein 1
Calculated molecular weight 14 kDa
Observed molecular weight 14 kDa
GenBank accession numberBC007090
Gene symbol HINT1
Gene ID (NCBI) 3094
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HINT1(Histidine triad nucleotide-binding protein 1) is also named as HINT, PKCI1, PRKCNH1,which is a member of the histidine triad (HIT) family, highly conserved in diverse species and ubiquitously expressed in mammalian tissues.It hydrolyzes adenosine 5'-monophosphoramidate substrates such as AMP-morpholidate, AMP-N-alanine methyl ester, AMP-alpha-acetyl lysine methyl ester and AMP-NH2.This antibody is specific to HINT1.


Product Specific Protocols
WB protocol for HINT1 antibody 10717-1-APDownload protocol
IHC protocol for HINT1 antibody 10717-1-APDownload protocol
IP protocol for HINT1 antibody 10717-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Nat Commun

Second messenger Ap4A polymerizes target protein HINT1 to transduce signals in FcεRI-activated mast cells.

Authors - Jing Yu


Post-translational modification of HINT1 mediates activation of MITF transcriptional activity in human melanoma cells.

Authors - A Motzik

J Med Chem

A Chemical Strategy for Intracellular Arming of an Endogenous Broad-Spectrum Antiviral Nucleotide

Authors - Kellan T. Passow

Cell Cycle

The tumor suppressor HINT1 regulates MITF and β-catenin transcriptional activity in melanoma cells.

Authors - Genovese Giannicola G

Pharmacogenomics J

Association of the histidine-triad nucleotide-binding protein-1 (HINT1) gene variants with nicotine dependence.

Authors - Jackson K J KJ

J Proteome Res

Novel breast cancer metastasis-associated proteins.

Authors - Ho Jiapei J