CoraLite® Plus 488-conjugated HIC2 Recombinant antibody

HIC2 Recombinant Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

230177E8

Cat no : CL488-83162-7

Synonyms

Hic-3, Hic-2, HIC1-related gene on chromosome 22 protein, Hic 3, Hic 2

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Validation Data Gallery

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  IF Staining of HepG2 using CL488-83162-7

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 HIC2 antibody (CL488-83162-7, Clone: 230177E8 ) at dilution of 1:200.

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  FC experiment of HepG2 using CL488-83162-7

  1x10^6 HepG2 cells were intracellularly stained with 0.4 ug Coralite® Plus 488 Hic2 Recombinant Antibody (CL488-83162-7, Clone:230177E8)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL488-83162-7 is HIC2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: HIC2 fusion protein Ag18789
• Full Name: hypermethylated in cancer 2
• Calculated Molecular Weight: 615 aa, 66 kDa
• GenBank Accession Number: BC094787
• Gene Symbol: HIC2
• Gene ID (NCBI): 23119
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage.

Background Information

HIC2 (hypermethylated in cancer 2) is a transcription factor related to the tumor suppressor HIC1, required for normal cardiac development and lost in distal variants of 22q11 Deletion Syndrome (PMID: 29061339). HIC2 is also a POK family protein, and the amino acid sequence of its BTB/POZ and zinc-finger domains are similar to those of HIC1(PMID: 31127867). Studies have shown that HIC2 plays a role in suppressing fetal genes for normal development of the heart and circulatory system, in embryogenesis (PMID: 24748541).