HIBCH Recombinant antibody, PBS Only (Detector)

HIBCH Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240590A3

Cat no : 83569-1-PBS

Synonyms

HIB-CoA hydrolase, HIB CoA hydrolase, EC:3.1.2.4, 3-hydroxyisobutyryl-coenzyme A hydrolase, 3-hydroxyisobutyryl-CoA hydrolase, mitochondrial

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Validation Data Gallery

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  WB analysis using 83569-1-RR (same clone as 83569-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83569-1-RR (HIBCH antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83569-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 83569-1-RR (same clone as 83569-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HIBCH antibody (83569-1-RR, Clone: 240590A3 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 83569-1-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 83569-1-RR (same clone as 83569-1-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug HIBCH Recombinant antibody (83569-1-RR, Clone:240590A3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83569-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00560-3

  Cytometric bead array standard curve of MP00560-3, HIBCH Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83569-3-PBS. Detection antibody: 83569-1-PBS. Standard: Ag6149. Range: 0.625-80 ng/mL.

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  Affinity and Kinetic Characterization of 83569-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83569-1-RR against Human HIBCH were performed. The affinity constant is 1.37 nM.

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Product Information

83569-1-PBS targets HIBCH as part of a matched antibody pair:

MP00560-3: 83569-3-PBS capture and 83569-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: HIBCH fusion protein Ag6149
• Full Name: 3-hydroxyisobutyryl-Coenzyme A hydrolase
• Calculated Molecular Weight: 43 kDa
• GenBank Accession Number: BC067822
• Gene Symbol: HIBCH
• Gene ID (NCBI): 26275
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.