HEY2 Polyclonal antibody

• Featured Product
• KD/KO Validated

HEY2 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, IP, CoIP, chIP, ELISA

Conjugate

Unconjugated

Publications

45

Reviews (3)

Cat no : 10597-1-AP

Synonyms

GRL, GRIDLOCK, Class B basic helix-loop-helix protein 32, CHF1, Cardiovascular helix-loop-helix factor 1

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Validation Data Gallery

• 

  WB analysis of K-562 using 10597-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10597-1-AP (HEY2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 10597-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 10597-1-AP (HEY2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of 4T1 using 10597-1-AP

  4T1 cells were subjected to SDS PAGE followed by western blot with 10597-1-AP (HEY2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 10597-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10597-1-AP (HEY2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 10597-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 10597-1-AP (HEY2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse heart using 10597-1-AP

  IP result of anti-HEY2 (IP:10597-1-AP, 5ug; Detection:10597-1-AP 1:500) with mouse heart tissue lysate 7500ug.

• 

  IF Staining of HepG2 using 10597-1-AP

  Immunofluorescent analysis of HepG2 cells, using HEY2 antibody 10597-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

• Positive WB detected in: K-562 cells, mouse heart tissue, MCF-7 cells, 4T1 cells
• Positive IP detected in: mouse heart tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 26 publications below
• IHC: See 9 publications below
• IF: See 9 publications below
• CoIP: See 1 publications below
• ChIP: See 2 publications below

Product Information

10597-1-AP targets HEY2 in WB, IHC, IF, IP, CoIP, chIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HEY2 fusion protein Ag0913
• Full Name: hairy/enhancer-of-split related with YRPW motif 2
• Calculated Molecular Weight: 36 kDa
• Observed Molecular Weight: 33-40 kDa
• GenBank Accession Number: BC007707
• Gene Symbol: HEY2
• Gene ID (NCBI): 23493
• RRID: AB_2118415
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Hairy/enhancer of split-related proteins, such as HEY2, are basic helix-loop-helix (bHLH) transcription factors implicated in cell fate decision and boundary formation. HEY genes are direct transcriptional targets of the Notch signaling pathways in Drosophila and vertebrates. HEY2 is similarly expressed in the somites whereas it shows a complementary expression in the heart, the craniofacial region and the nervous system. This antibody is a rabbit polyclonal antibody raised against full length of human HEY2.

Publications

Species	Application	Title
human	IF	Cell; Multi-chamber cardioids unravel human heart development and cardiac defects; Authors - Clara Schmidt
human	IF	Cell Stem Cell; Generation of Functional Liver Sinusoidal Endothelial Cells from Human Pluripotent Stem-Cell-Derived Venous Angioblasts.; Authors - Blair K Gage
mouse	WB	Nat Neurosci; Zeb2 recruits HDAC-NuRD to inhibit Notch and controls Schwann cell differentiation and remyelination.; Authors - Lai Man Natalie Wu
human	chIP	Nat Commun; Defining human cardiac transcription factor hierarchies using integrated single-cell heterogeneity analysis.; Authors - Jared M Churko
human	IF	Nat Commun; Generation of mature compact ventricular cardiomyocytes from human pluripotent stem cells.; Authors - Shunsuke Funakoshi
human	WB	Nat Commun; Cathepsin K-mediated notch1 activation contributes to neovascularization in response to hypoxia.; Authors - Haiying Jiang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

CHAO (Verified Customer) (03-31-2022)

Weak band around correct size.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse Heart

FH

Tom (Verified Customer) (08-22-2020)

I performed western blot with mouse heart tissue lysate using Hey2 antibody. A specific ~36 kDa Hey2 band was observed.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse heart

FH

Tom (Verified Customer) (08-22-2020)

This Hey2 antibody is highly recommended for detecting Hey2 on frozen tissue sections! I used this Hey2 antibody for immunofluorescence (IF). It clearly had nuclear staining signal in compact myocardium of embryonic heart, which is consistent with Hey2's well-known expression pattern.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Mouse embryonic heart

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