HEY1 Polyclonal antibody

• Featured Product
• KD/KO Validated

HEY1 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Publications

52

Reviews (1)

Cat no : 19929-1-AP

Synonyms

HESR 1, HERP2, CHF-2, CHF2, CHF 2

---

Validation Data Gallery

• 

  WB analysis using 19929-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19929-1-AP (HEY1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 19929-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 19929-1-AP (HEY1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse kidney using 19929-1-AP

  IP result of anti-HEY1 (IP:19929-1-AP, 4ug; Detection:19929-1-AP 1:500) with mouse kidney tissue lysate 4400ug.

---

Tested Applications

• Positive WB detected in: mouse testis tissue, A549 cells, NCI-H1299 cells, rat testis tissue
• Positive IP detected in: mouse kidney tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 46 publications below
• IHC: See 2 publications below
• IF: See 3 publications below
• IP: See 1 publications below
• FC: See 1 publications below
• CoIP: See 1 publications below
• ChIP: See 4 publications below

Product Information

19929-1-AP targets HEY1 in WB, IHC, IF, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HEY1 fusion protein Ag13906
• Full Name: hairy/enhancer-of-split related with YRPW motif 1
• Calculated Molecular Weight: 304 aa, 33 kDa
• Observed Molecular Weight: 32-34 kDa
• GenBank Accession Number: BC001873
• Gene Symbol: HEY1
• Gene ID (NCBI): 23462
• RRID: AB_10646438
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Hairy/enhancer of split-related proteins are basic helix-loop-helix (bHLH) transcription factors implicated in cell fate decision and boundary formation. And HEY1 is one of such protein. It is the direct downstream effector of Notch signaling which may be required for cardiovascular development. It preferentially bind to the canonical E box sequence 5'-CACGTG-3' and acts as a transcriptional repressor. HEY1 is a 33kDa protein and forms dimer by self-associating.

Publications

Species	Application	Title
human	IHC	Protein Cell; Microbiota enterotoxigenic Bacteroides fragilis-secreted BFT-1 promotes breast cancer cell stemness and chemoresistance through its functional receptor NOD1; Authors - Wei Ma
mouse	WB	Theranostics; Nicotinamide mononucleotide enhances fracture healing by promoting skeletal stem cell proliferation; Authors - Yitian Shi
human	WB	J Exp Clin Cancer Res; ACTN1 promotes HNSCC tumorigenesis and cisplatin resistance by enhancing MYH9-dependent degradation of GSK-3β and integrin β1-mediated phosphorylation of FAK; Authors - Li Cui
mouse	WB	Dev Cell; Regulation of reverse electron transfer at mitochondrial complex I by unconventional Notch action in cancer stem cells.; Authors - Rani Ojha
human	WB	Nat Commun; Cathepsin K-mediated notch1 activation contributes to neovascularization in response to hypoxia.; Authors - Haiying Jiang
	WB	Cell Rep; Akt3 activation by R-Ras in an endothelial cell enforces quiescence and barrier stability of neighboring endothelial cells via Jagged1; Authors - Jose Luis Herrera

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Zhenminm (Verified Customer) (07-10-2019)

It is too much non-specific background.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1,000
• Cell Tissue Type: Mouse Testis

HEY1 Antibody Western Blot, validation (1:1,000 dilution) in Mouse Testis (Cat no:19929-1-AP)