Anti-Mouse HER2/ErbB2 Rabbit Recombinant Antibody, PBS Only

HER2/ErbB2 Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Conjugate

Unconjugated

CloneNo.

241366E6

Cat no : 98153-1-PBS

Synonyms

Her2 / ErbB2, Her2, Erbb2, EC:2.7.10.1, CD340

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Validation Data Gallery

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  FC experiment of NIH/3T3 using 98153-1-RR (same clone as 98153-1-PBS)

  1x10^6 NIH/3T3 cells were surface stained with 0.25 ug Anti-Mouse HER2/ErbB2 Rabbit Recombinant Antibody (98153-1-RR, Clone: 241366E6) (red) or Isotype Control (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98153-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 98153-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98153-1-RR against Mouse HER2/ErbB2 were performed. The affinity constant is 1.50 nM.

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Product Information

The immunogen of 98153-1-PBS is HER2/ErbB2 Fusion Protein expressed in E. coli.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)
• Calculated Molecular Weight: 139 kDa
• GenBank Accession Number: NM_001003817.1
• Gene Symbol: Erbb2
• Gene ID (NCBI): 13866
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

HER2, also known as ErbB2, Neu, and CD340, is a 185-kDa transmembrane glycoprotein member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. It has no ligand-binding domain and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signaling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase.