HELT Polyclonal antibody

HELT Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 24730-1-AP

Synonyms

HCM1228, HELT, HESL, Mgn

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Validation Data Gallery

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  WB analysis of rat heart using 24730-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 24730-1-AP (HELT Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 24730-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 24730-1-AP (HELT Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: rat heart tissue, mouse heart tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

24730-1-AP targets HELT in WB, ELISA applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HELT fusion protein Ag20618
• Full Name: HES/HEY-like transcription factor
• Calculated Molecular Weight: 327 aa, 36 kDa
• Observed Molecular Weight: 27 kDa
• GenBank Accession Number: BC140795
• Gene Symbol: HELT
• Gene ID (NCBI): 391723
• RRID: AB_2879694
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HELT is a transcriptional repressor which binds preferentially to the canonical E box sequence 5'-CACGCG-3'. HELT has two isoforms with MW 36 kDa and 27 kDa.