HDAC4-specific Polyclonal antibody

• Featured Product
• KD/KO Validated

HDAC4-specific Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF-P, IP, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 16165-1-AP

Synonyms

HDAC4, KIAA0288, HD4, EC:3.5.1.98

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Validation Data Gallery

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  WB analysis using 16165-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16165-1-AP (HDAC4-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 16165-1-AP

  IP result of anti-HDAC4-specific (IP:16165-1-AP, 4ug; Detection:16165-1-AP 1:500) with HeLa cells lysate 2440ug.

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  IHC staining of human ovary cancer using 16165-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 16165-1-AP (HDAC4-specific antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 16165-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16165-1-AP (HDAC4-specific Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human ovary cancer using 16165-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 16165-1-AP (HDAC4-specific antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 16165-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16165-1-AP (HDAC4-specific Antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of mouse liver using 16165-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using HDAC4-specific antibody (16165-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human ovary cancer tissue, human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 1 publications below

Product Information

16165-1-AP targets HDAC4-specific in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: histone deacetylase 4
• Calculated Molecular Weight: 119 kDa
• Observed Molecular Weight: 119-140 kDa
• GenBank Accession Number: BC039904
• Gene Symbol: HDAC4
• Gene ID (NCBI): 9759
• RRID: AB_2279763
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HDAC4, also named as HDACA, belongs to the histone deacetylase family. HDAC4 is responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. HDAC4 is Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D. This antibody is a rabbit polyclonal antibody raised against a peptide mapping within human HDAC4.

Publications

Species	Application	Title
human,mouse	IF	Nat Cell Biol; Persistence of a regeneration-associated, transitional alveolar epithelial cell state in pulmonary fibrosis.; Authors - Yoshihiko Kobayashi
human	WB	Hepatology; The histone deacetylase 4/SP1/microrna-200a regulatory network contributes to aberrant histone acetylation in hepatocellular carcinoma.; Authors - Yuan Ji-hang JH ; KD Validated
mouse	WB	J Orthop Translat; Irisin reduces bone fracture by facilitating osteogenesis and antagonizing TGF-β/Smad signaling in a growing mouse model of osteogenesis imperfecta; Authors - Bin Sun
mouse	WB	Biol Trace Elem Res; Exercise Alleviates Fluoride-Induced Learning and Memory Impairment in Mice: Role of miR-206-3p and PREG; Authors - Lei Chai