HDAC4 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

HDAC4 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D2G12

Cat no : 66838-1-PBS

Synonyms

KIAA0288, HD4, EC:3.5.1.98, 1D2G12

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Validation Data Gallery

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  WB analysis using 66838-1-Ig (same clone as 66838-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66838-1-Ig (HDAC4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66838-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66838-1-Ig (same clone as 66838-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66838-1-Ig (HDAC4 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66838-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66838-1-Ig (same clone as 66838-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66838-1-Ig (HDAC4 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66838-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse liver using 66838-1-Ig (same clone as 66838-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using HDAC4 antibody (66838-1-Ig, Clone: 1D2G12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66838-1-PBS in a different storage buffer formulation.

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  IF Staining of C2C12 using 66838-1-Ig (same clone as 66838-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using HDAC4 antibody (66838-1-Ig, Clone: 1D2G12 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 66838-1-PBS in a different storage buffer formulation.

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Product Information

66838-1-PBS targets HDAC4 in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: HDAC4 fusion protein Ag11345
• Full Name: histone deacetylase 4
• Calculated Molecular Weight: 972 aa, 106 kDa
• Observed Molecular Weight: 140 kDa
• GenBank Accession Number: BC039904
• Gene Symbol: HDAC4
• Gene ID (NCBI): 9759
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

HDAC4, also named as HDACA, belongs to the histone deacetylase family. HDAC4 is responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. HDAC4 is Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D.