HDAC4 Polyclonal antibody

HDAC4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 29252-1-AP

Synonyms

HA6116, HD4, HDAC, HDAC A, HDAC4, HDACA, histone deacetylase 4, KIAA0288

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Validation Data Gallery

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  WB analysis using 29252-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29252-1-AP (HDAC4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 29252-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 29252-1-AP (HDAC4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 29252-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 29252-1-AP (HDAC4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 29252-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 29252-1-AP (HDAC4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 29252-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 29252-1-AP (HDAC4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HEK-293T cells, HeLa cells, Jurkat cells
• Positive IHC detected in: human ovary tumor tissue, human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

29252-1-AP targets HDAC4 in WB, IHC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HDAC4 fusion protein Ag30752
• Full Name: histone deacetylase 4
• Calculated Molecular Weight: 972 aa, 106 kDa
• Observed Molecular Weight: 120-140 kDa
• GenBank Accession Number: BC039904
• Gene Symbol: HDAC4
• Gene ID (NCBI): 9759
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HDAC4 (Histone deacetylase 4) belongs to class II of the histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. Histone deacetylation gives a tag for epigenetic repression and plays a role in transcriptional regulation, cell cycle progression and developmental events. It is involved in muscle maturation by its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D. HDAC4 is required for TGFbeta1-induced myofibroblastic differentiation (PMID: 17610967). HDAC4 encoded by an allele associated with mental retardation is a gain-of-function nuclear repressor that abolishes transcription and synaptic transmission (PMID: 23141539).