HDAC10 Polyclonal antibody

HDAC10 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 24913-1-AP

Synonyms

HD10, HDAC10, histone deacetylase 10

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Validation Data Gallery

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  WB analysis of fetal human brain using 24913-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 24913-1-AP (HDAC10 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 24913-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 24913-1-AP (HDAC10 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 24913-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24913-1-AP (HDAC10 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon cancer using 24913-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24913-1-AP (HDAC10 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human colon cancer using 24913-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24913-1-AP (HDAC10 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 24913-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24913-1-AP (HDAC10 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of MCF-7 using 24913-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using HDAC10 antibody (24913-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

• Positive WB detected in: fetal human brain tissue, mouse thymus tissue
• Positive IHC detected in: human colon cancer tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IHC: See 3 publications below

Product Information

24913-1-AP targets HDAC10 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HDAC10 fusion protein Ag18470
• Full Name: histone deacetylase 10
• Calculated Molecular Weight: 669 aa, 71 kDa
• Observed Molecular Weight: 68 kDa
• GenBank Accession Number: BC125083
• Gene Symbol: HDAC10
• Gene ID (NCBI): 83933
• RRID: AB_2879793
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HDAC10, also named as Histone deacetylase 10, is a 669 amino acid protein, which is widely expressed with high levels in liver and kidney. HDAC10, which belongs to the class IIb subfamily, has an active deacetylase domain (DAC) and a catalytically inactive leucine-rich domain (LRD). HDAC10 is determined that it plays a role in homologous recombination, promotes autophagy and survival in neuroblastoma cells, suppresses cervical cancer metastasis and facilitates the cell cycle.

Publications

Species	Application	Title
mouse, rat	WB	Kidney Int; Histone deacetylase 4 selectively contributes to podocyte injury in diabetic nephropathy.; Authors - Xiaojie Wang
human	IHC	Oncol Lett; HDAC10 expression is associated with DNA mismatch repair gene and is a predictor of good prognosis in colon carcinoma.; Authors - Xiangxiang Tao
human	WB	Cell Death Dis; WNT5A promotes the metastasis of esophageal squamous cell carcinoma by activating the HDAC7/SNAIL signaling pathway.; Authors - Yingtong Feng
human	IHC,WB	J Cell Mol Med; RNA m5C methylation orchestrates BLCA progression via macrophage reprogramming; Authors - Dali Yan
human	WB,IHC	Discov Oncol; Elevated histone deacetylase 10 expression promotes the progression of clear cell renal cell carcinoma by Notch-1-PTEN signaling axis; Authors - Bin Zheng