HDAC1-specific Polyclonal antibody

HDAC1-specific Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 16160-1-AP

Synonyms

GON 10, HD1, HDAC1, histone deacetylase 1, RPD3, RPD3L1

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Validation Data Gallery

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  WB analysis of mouse testis using 16160-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 16160-1-AP (HDAC1-specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 16160-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 16160-1-AP (HDAC1-specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 16160-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 16160-1-AP (HDAC1-specific antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 16160-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16160-1-AP (HDAC1-specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse testis using 16160-1-AP

  IP result of anti-HDAC1-specific (IP:16160-1-AP, 4ug; Detection:16160-1-AP 1:500) with mouse testis tissue lysate 4000ug.

• 

  IF Staining of HepG2 using 16160-1-AP

  Immunofluorescent analysis of HepG2 cells using 16160-1-AP (HDAC1-specific antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Tested Applications

• Positive WB detected in: mouse testis tissue, mouse brain tissue, mouse thymus tissue
• Positive IP detected in: mouse testis tissue
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16160-1-AP targets HDAC1-specific in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: histone deacetylase 1
• Calculated Molecular Weight: 55 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: NM_004964
• Gene Symbol: HDAC1
• Gene ID (NCBI): 3065
• RRID: AB_2118060
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Histone deacetylases(HDAC) are a class of enzymes that remove the acetyl groups from the lysine residues leading to the formation of a condensed and transcriptionally silenced chromatin. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex, which is responsible for gene expression silencing. It also plays an important role in the control of cell proliferation and differentiation by interacting with RB, p53 and other transcription factors. At least 4 classes of HDAC were identified. As a class I HDAC, HDAC 1 was primarily found in the nucleus. This antibody is a rabbit polyclonal antibody raised against a peptide mapping near the N-terminus of human HDAC1 and is specific to HDAC1. It does not react with other HDACs.