HDAC1 Monoclonal antibody, PBS Only

HDAC1 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4C4G7

Cat no : 66085-1-PBS

Synonyms

GON 10, HD1, HDAC, HDAC1, histone deacetylase 1, RPD3, RPD3L1

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Validation Data Gallery

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  WB analysis using 66085-1-Ig (same clone as 66085-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66085-1-Ig (HDAC1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 66085-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66085-1-Ig (same clone as 66085-1-PBS)

  Western blot analysis of HDAC1 in various cell lines using Proteintech antibody 66085-1-Ig at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66085-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 66085-1-Ig (same clone as 66085-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66085-1-Ig (HDAC1 Antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66085-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66085-1-Ig (same clone as 66085-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66085-1-Ig (HDAC1 Antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66085-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66085-1-Ig (same clone as 66085-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66085-1-Ig (HDAC1 Antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66085-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 66085-1-Ig (same clone as 66085-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66085-1-Ig (HDAC1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66085-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 66085-1-Ig (same clone as 66085-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66085-1-Ig (HDAC1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66085-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lymphoma using 66085-1-Ig (same clone as 66085-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66085-1-Ig (HDAC1 Antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66085-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lymphoma using 66085-1-Ig (same clone as 66085-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66085-1-Ig (HDAC1 Antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66085-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lymphoma using 66085-1-Ig (same clone as 66085-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66085-1-Ig (HDAC1 Antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66085-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 66085-1-Ig (same clone as 66085-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using HDAC1 antibody (66085-1-Ig, Clone: 4C4G7 ) at dilution of 1:12000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66085-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A431 using 66085-1-Ig (same clone as 66085-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using HDAC1 antibody (66085-1-Ig, Clone: 4C4G7 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66085-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 66085-1-Ig (same clone as 66085-1-PBS)

  Immunofluorescent analysis of (10% Formaldehyde ) fixed HeLa cells using 66085-1-Ig(HDAC1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66085-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66085-1-PBS targets HDAC1 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: HDAC1 fusion protein Ag19128
• Full Name: histone deacetylase 1
• Calculated Molecular Weight: 55 kDa
• Observed Molecular Weight: 65 kDa
• GenBank Accession Number: BC000301
• Gene Symbol: HDAC1
• Gene ID (NCBI): 3065
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Histone deacetylases(HDAC) are a class of enzymes that remove the acetyl groups from the lysine residues leading to the formation of a condensed and transcriptionally silenced chromatin. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex, which is responsible for gene expression silencing. It also plays an important role in the control of cell proliferation and differentiation by interacting with RB, p53 and other transcription factors. At least 4 classes of HDAC were identified. As a class I HDAC, HDAC 1 was primarily found in the nucleus. This antibody is raised against residues near the C terminus of human HDAC1. The calculated molecular weight of HDAC1 is 55 kDa, but modified HDAC1 is about 60-65 kDa .