HDAC Assay Kits

Synonyms

histone, histone deacetylase, deacetylase, acetylation, histone deacetylation, histone modification, HDAC, HDAC assay, sirtuins, fluorescent

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Overview

HDACs, histone deacetylases, remove acetyl groups from lysine residues contained in both histone and non-histone proteins. HDACs act in direct opposition to histone acetylases (HATs). The balance of HDAC and HAT activity determines the acetylation state of lysines on proteins throughout the cell. These acetyl groups affect chromatin structure, protein stability, protein-protein interactions, gene regulation, and more. Disease models that exhibit open chromatin transitioning to closed chromatin, increased protein degradation, differential protein interacting partners, or simply show differences in gene expression, are all candidates for HDAC analysis.

HDAC Assay Kits are an easy and sensitive way to determine HDAC activity in your samples (e.g. disease vs. normal, HDACi drug screens, purified HDAC enzymes). This assay utilizes a short peptide substrate that contains acetylated lysine residues. Once the residue is deacetylated, the lysine residue interacts with the developing solution to produce a signal. Kits also include positive control HeLa nuclear extract, deacetylated HDAC assay standard, HDAC peptide substrate, and Trichostatin A as a model inhibitor. Our 96-well plate format is available in both colorimetric and fluorescent formats to suit your needs.

To learn more about our HDAC Assay Kits, click on the Data or Contents tabs below. To view a manual or other related documents, click on the Documents tab below.

Data

Figure 1: Colorimetric HDAC Assay results.HeLa nuclear extracts were assayed from 0 to 50 µg per well in duplicate. The purple line represents activity from untreated extracts, while the copper line represents extracts treated with 1 µM Trichostatin A inhibitor.

Figure 2: Fluorescent HDAC Assay results.HeLa nuclear extracts were assayed from 0 to 10 µg per well in duplicate. The purple line represents activity from untreated extracts, while the copper line represents extracts treated with 1 µM Trichostatin A inhibitor.

Contents

Contents & Storage

HDAC Assay Buffer, HDAC Substrate, HDAC Assay Standard, Trichostatin A, HDAC Developer Solution, HeLa Nuclear Extract, 96-well half-volume plate. HeLa Nuclear Extract is to be stored at -80°C. Store all other components at -20°C. All reagents are guaranteed stable for 6 months from date of receipt when stored properly.

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